Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 893275..893799 Replicon chromosome
Accession NZ_CP016072
Organism Staphylococcus pseudintermedius strain NA45 isolate UWMNA45

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag A9I66_RS04055 Protein ID WP_014613379.1
Coordinates 893446..893799 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag A9I66_RS04050 Protein ID WP_014613378.1
Coordinates 893275..893445 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9I66_RS04025 889164..889637 + 474 WP_014613373.1 PH domain-containing protein -
A9I66_RS04030 889630..891159 + 1530 WP_065354118.1 PH domain-containing protein -
A9I66_RS04035 891143..891661 + 519 WP_065354119.1 PH domain-containing protein -
A9I66_RS04040 891658..892008 + 351 WP_014613376.1 holo-ACP synthase -
A9I66_RS04045 892042..893190 + 1149 WP_065354670.1 alanine racemase -
A9I66_RS04050 893275..893445 + 171 WP_014613378.1 hypothetical protein Antitoxin
A9I66_RS04055 893446..893799 + 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A9I66_RS04060 893857..894861 + 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
A9I66_RS04065 894940..895266 + 327 WP_014613381.1 anti-sigma factor antagonist -
A9I66_RS04070 895266..895745 + 480 WP_031253870.1 anti-sigma B factor RsbW -
A9I66_RS04075 895720..896490 + 771 WP_015729428.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T64205 WP_014613379.1 NZ_CP016072:893446-893799 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T64205 NZ_CP016072:893446-893799 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATTCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT64205 WP_014613378.1 NZ_CP016072:893275-893445 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT64205 NZ_CP016072:893275-893445 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References