Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 878360..878881 Replicon chromosome
Accession NZ_CP016072
Organism Staphylococcus pseudintermedius strain NA45 isolate UWMNA45

Toxin (Protein)


Gene name TsdT Uniprot ID A0A3D8YLE8
Locus tag A9I66_RS03980 Protein ID WP_014613364.1
Coordinates 878660..878881 (+) Length 74 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A166R1D0
Locus tag A9I66_RS03975 Protein ID WP_014613363.1
Coordinates 878360..878647 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9I66_RS03950 873621..874424 + 804 WP_014613358.1 hydroxyethylthiazole kinase -
A9I66_RS03955 874414..875052 + 639 WP_014613359.1 thiamine phosphate synthase -
A9I66_RS03960 875141..876007 + 867 WP_014613360.1 membrane protein insertase YidC -
A9I66_RS03965 876061..876717 - 657 WP_014613361.1 HD domain-containing protein -
A9I66_RS03970 876738..878228 - 1491 WP_014613362.1 cardiolipin synthase -
A9I66_RS03975 878360..878647 + 288 WP_014613363.1 copper-sensing transcriptional repressor CsoR Antitoxin
A9I66_RS03980 878660..878881 + 222 WP_014613364.1 heavy-metal-associated domain-containing protein Toxin
A9I66_RS03985 878885..879091 + 207 WP_014613365.1 cation transporter -
A9I66_RS03990 879192..881372 + 2181 WP_065354117.1 heavy metal translocating P-type ATPase -
A9I66_RS14395 881458..881604 + 147 WP_014613367.1 hypothetical protein -
A9I66_RS04000 882572..883300 + 729 WP_014613368.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(21-64)

Antitoxin

Metal-sensitive transcriptional repressor

(3-93)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8065.10 Da        Isoelectric Point: 4.3480

>T64204 WP_014613364.1 NZ_CP016072:878660-878881 [Staphylococcus pseudintermedius]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGFPVIQSHKGV

Download         Length: 222 bp

>T64204 NZ_CP016072:878660-878881 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10997.59 Da        Isoelectric Point: 7.0504

>AT64204 WP_014613363.1 NZ_CP016072:878360-878647 [Staphylococcus pseudintermedius]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Download         Length: 288 bp

>AT64204 NZ_CP016072:878360-878647 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3D8YLE8

Antitoxin

Source ID Structure
AlphaFold DB A0A166R1D0

References