Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 971404..971890 Replicon chromosome
Accession NZ_CP016027
Organism Halothiobacillus sp. LS2

Toxin (Protein)


Gene name - Uniprot ID A0A191ZFQ0
Locus tag A9404_RS04335 Protein ID WP_066099021.1
Coordinates 971627..971890 (-) Length 88 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A191ZFS1
Locus tag A9404_RS04330 Protein ID WP_066099020.1
Coordinates 971404..971637 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9404_RS04300 966832..967968 + 1137 WP_066102776.1 glycerate kinase -
A9404_RS04305 967999..968703 - 705 WP_066099011.1 VIT family protein -
A9404_RS13330 968782..968934 + 153 WP_156521229.1 hypothetical protein -
A9404_RS04310 969018..969524 + 507 WP_066102779.1 DUF2127 domain-containing protein -
A9404_RS04315 969545..970018 - 474 WP_066099013.1 DUF3592 domain-containing protein -
A9404_RS04320 970096..970512 - 417 WP_066099016.1 VOC family protein -
A9404_RS04325 970630..971013 - 384 WP_066099018.1 SgcJ/EcaC family oxidoreductase -
A9404_RS04330 971404..971637 - 234 WP_066099020.1 CopG family transcriptional regulator Antitoxin
A9404_RS04335 971627..971890 - 264 WP_066099021.1 BrnT family toxin Toxin
A9404_RS13335 972001..972387 - 387 WP_156521230.1 hypothetical protein -
A9404_RS04340 972517..972867 - 351 WP_066099023.1 DUF3024 domain-containing protein -
A9404_RS04345 972975..973385 - 411 WP_066099024.1 lactoylglutathione lyase -
A9404_RS04350 973504..974304 - 801 WP_066099026.1 arsenite methyltransferase -
A9404_RS04355 974393..974941 - 549 WP_197490429.1 RNA polymerase sigma factor SigZ -
A9404_RS04360 976061..976062 - 2 WP_156521231.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 971404..991554 20150
- inside Integron - - 971044..991554 20510


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10078.36 Da        Isoelectric Point: 7.2032

>T64160 WP_066099021.1 NZ_CP016027:c971890-971627 [Halothiobacillus sp. LS2]
MAAFEYDERKSQSNRKKHGIDFVEAQGLWNDPYLVEIAARTTEEARFLVIGKIQEKHWSAVVTPRDGNIRIISVRRSRAE
EVAIYES

Download         Length: 264 bp

>T64160 NZ_CP016027:c971890-971627 [Halothiobacillus sp. LS2]
ATGGCTGCATTTGAGTACGACGAACGGAAAAGTCAGAGCAACCGGAAAAAGCACGGGATCGACTTCGTCGAGGCTCAAGG
GCTTTGGAATGACCCGTATCTTGTTGAAATTGCAGCGAGAACGACGGAAGAGGCGCGTTTCTTGGTGATCGGAAAGATCC
AAGAAAAGCACTGGTCAGCGGTTGTCACGCCTCGCGACGGCAATATCCGAATTATCTCGGTCCGTCGCTCCCGAGCAGAA
GAGGTAGCGATTTATGAAAGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8942.27 Da        Isoelectric Point: 10.0102

>AT64160 WP_066099020.1 NZ_CP016027:c971637-971404 [Halothiobacillus sp. LS2]
MKAKSFDAKFDKGEDVTDVLDLSKARRPLQEQRRVNVDFPTWMIESLDREAGRLGVTRQSVIKIWLAERLEQIAPNK

Download         Length: 234 bp

>AT64160 NZ_CP016027:c971637-971404 [Halothiobacillus sp. LS2]
ATGAAAGCTAAAAGTTTCGACGCGAAATTTGACAAAGGAGAGGATGTCACCGACGTCCTCGACCTGTCCAAGGCTCGCCG
GCCTCTCCAGGAACAACGCCGGGTGAACGTGGATTTTCCCACCTGGATGATCGAGTCCCTTGATCGAGAAGCTGGTCGTT
TGGGGGTCACTCGCCAGTCAGTGATCAAAATATGGCTCGCCGAGCGCTTAGAGCAGATTGCTCCTAACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A191ZFQ0

Antitoxin

Source ID Structure
AlphaFold DB A0A191ZFS1

References