Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-PumB/COG3657-dnstrm_HI1420
Location 1559939..1560543 Replicon chromosome
Accession NZ_CP016023
Organism Ralstonia insidiosa strain ATCC 49129

Toxin (Protein)


Gene name relE Uniprot ID S9TBH7
Locus tag A9Y76_RS25405 Protein ID WP_021193105.1
Coordinates 1560244..1560543 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A192A5P2
Locus tag A9Y76_RS25400 Protein ID WP_064808637.1
Coordinates 1559939..1560241 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9Y76_RS25375 1555491..1557110 + 1620 WP_064808628.1 AMP-binding protein -
A9Y76_RS25380 1557107..1557523 + 417 WP_064808630.1 acyl-CoA thioesterase -
A9Y76_RS25385 1557549..1557941 + 393 WP_064808632.1 RidA family protein -
A9Y76_RS25390 1557991..1558281 - 291 WP_064809116.1 hypothetical protein -
A9Y76_RS25395 1558436..1559740 - 1305 WP_064808634.1 metabolite/H+ symporter -
A9Y76_RS25400 1559939..1560241 - 303 WP_064808637.1 putative addiction module antidote protein Antitoxin
A9Y76_RS25405 1560244..1560543 - 300 WP_021193105.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A9Y76_RS25410 1560642..1561871 - 1230 WP_064808639.1 MFS transporter -
A9Y76_RS25415 1562050..1562838 - 789 Protein_1386 GntR family transcriptional regulator -
A9Y76_RS25420 1563029..1563571 - 543 WP_031328796.1 helix-turn-helix transcriptional regulator -
A9Y76_RS25425 1563704..1564282 + 579 WP_064808641.1 GNAT family N-acetyltransferase -
A9Y76_RS25430 1564530..1564973 + 444 WP_021193100.1 VOC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-85)

Antitoxin

Cro/C1-type helix-turn-helix domain

(52-95)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11245.93 Da        Isoelectric Point: 9.7981

>T64157 WP_021193105.1 NZ_CP016023:c1560543-1560244 [Ralstonia insidiosa]
MLTIRTTEVFDAWFAKLRDKTAQRRIQVRIDRLQLGNPGDAKAVRDGIGELRIDHGPGYRVYFVQRGAVLIILLCGGDKS
TQEADIRRAIDLSRHLDVE

Download         Length: 300 bp

>T64157 NZ_CP016023:c1560543-1560244 [Ralstonia insidiosa]
ATGCTGACGATCCGCACCACCGAAGTGTTCGATGCCTGGTTCGCGAAGCTGCGTGACAAGACTGCGCAGCGGCGCATCCA
GGTGCGCATCGACCGTCTGCAACTGGGCAATCCGGGCGATGCGAAGGCGGTGCGCGATGGCATCGGTGAGCTTCGGATCG
ATCATGGCCCCGGCTATCGCGTGTATTTCGTACAGCGTGGCGCGGTGCTGATCATCCTGCTTTGCGGCGGCGACAAGTCG
ACACAGGAAGCGGACATCCGGCGCGCGATCGACCTGTCGCGCCATCTCGACGTGGAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 10897.49 Da        Isoelectric Point: 5.6269

>AT64157 WP_064808637.1 NZ_CP016023:c1560241-1559939 [Ralstonia insidiosa]
MPTDKPVKTRLWDSAEHLKTEADMAEYLDACLEEAGDDPAFITHALGVVARARGMTQLARDTGMTREGLYKALSEEGNPS
FATVLKVMRALGIRLHAVPA

Download         Length: 303 bp

>AT64157 NZ_CP016023:c1560241-1559939 [Ralstonia insidiosa]
ATGCCGACCGACAAACCTGTCAAAACCCGCCTCTGGGACTCTGCCGAACACCTCAAAACCGAGGCCGACATGGCCGAGTA
TCTCGACGCTTGTCTGGAAGAAGCCGGCGATGACCCCGCCTTCATCACGCACGCGCTGGGCGTGGTTGCTCGTGCACGCG
GCATGACGCAACTCGCGCGTGACACTGGCATGACACGTGAAGGCCTCTACAAAGCACTGTCGGAAGAAGGCAATCCAAGC
TTCGCCACCGTGCTCAAGGTCATGCGTGCGTTGGGTATTCGGTTGCACGCTGTGCCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A117DRT1

Antitoxin

Source ID Structure
AlphaFold DB A0A192A5P2

References