Detailed information of TA system TA064146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3944622..3945436
• Replicon: chromosome
• Accession: NZ_CP016018
• Organism: Escherichia coli strain ER1821R

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: A9X67_RS19570
• Protein ID: WP_001054376.1
• Coordinates: 3944622..3944879 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: A9X67_RS19575
• Protein ID: WP_001309181.1
• Coordinates: 3944891..3945436 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X67_RS19545	3939910..3941016	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
A9X67_RS19550	3941081..3942061	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
A9X67_RS19555	3942644..3943884	-	1241	Protein_3737	helicase YjhR	-
A9X67_RS25525	3944000..3944245	+	246	Protein_3738	GNAT family N-acetyltransferase	-
A9X67_RS19570	3944622..3944879	+	258	WP_001054376.1	hypothetical protein	Toxin
A9X67_RS19575	3944891..3945436	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
A9X67_RS19580	3945492..3946238	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
A9X67_RS25055	3946407..3946625	+	219	Protein_3742	hypothetical protein	-
A9X67_RS25840	3946663..3946779	+	117	Protein_3743	VOC family protein	-
A9X67_RS19585	3947024..3948145	+	1122	WP_000010829.1	M42 family metallopeptidase	-
A9X67_RS19590	3948142..3948420	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
A9X67_RS19595	3948432..3949745	+	1314	WP_000460842.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	3933632..3953660	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T64146 WP_001054376.1 NZ_CP016018:3944622-3944879 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T64146 NZ_CP016018:3944622-3944879 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT64146 WP_001309181.1 NZ_CP016018:3944891-3945436 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT64146 NZ_CP016018:3944891-3945436 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8