Detailed information of TA system TA064143

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3596508..3597187
• Replicon: chromosome
• Accession: NZ_CP016018
• Organism: Escherichia coli strain ER1821R

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: A9X67_RS17855
• Protein ID: WP_000854672.1
• Coordinates: 3596846..3597187 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: A9X67_RS17850
• Protein ID: WP_000070395.1
• Coordinates: 3596508..3596825 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9X67_RS17810	3591555..3592418	+	864	WP_001065553.1	GTPase family protein	-
A9X67_RS17815	3592510..3593331	+	822	WP_000197389.1	DUF945 domain-containing protein	-
A9X67_RS25480	3593548..3593739	+	192	Protein_3405	DeoR family transcriptional regulator	-
A9X67_RS25485	3593735..3593962	+	228	WP_001548158.1	hypothetical protein	-
A9X67_RS25490	3593962..3594405	+	444	WP_000824223.1	hypothetical protein	-
A9X67_RS17830	3594428..3594895	+	468	WP_001547765.1	hypothetical protein	-
A9X67_RS17835	3594972..3595211	+	240	WP_000194654.1	DUF905 domain-containing protein	-
A9X67_RS17840	3595309..3595767	+	459	WP_000211838.1	antirestriction protein	-
A9X67_RS17845	3595783..3596259	+	477	WP_000811693.1	RadC family protein	-
A9X67_RS24165	3596268..3596489	+	222	WP_000691994.1	DUF987 domain-containing protein	-
A9X67_RS17850	3596508..3596825	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
A9X67_RS17855	3596846..3597187	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
A9X67_RS17865	3597759..3599012	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
A9X67_RS17870	3599024..3600127	-	1104	WP_001285288.1	glutamate 5-kinase	-
A9X67_RS17875	3600415..3601470	+	1056	WP_000749863.1	phosphoporin PhoE	-
A9X67_RS17880	3601509..3601910	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3562295..3597407	35112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T64143 WP_000854672.1 NZ_CP016018:3596846-3597187 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T64143 NZ_CP016018:3596846-3597187 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT64143 WP_000070395.1 NZ_CP016018:3596508-3596825 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT64143 NZ_CP016018:3596508-3596825 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684