Detailed information of TA system TA064114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 12902..13494
• Replicon: plasmid pNGF1_pCROD2_like
• Accession: NZ_CP016008
• Organism: Escherichia coli strain NGF1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: A9Z04_RS24560
• Protein ID: WP_000520549.1
• Coordinates: 12902..13150 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: A9Z04_RS24565
• Protein ID: WP_000681613.1
• Coordinates: 13147..13494 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9Z04_RS24515	8241..8492	+	252	WP_000121743.1	plasmid stabilization protein	-
A9Z04_RS24520	8482..8763	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A9Z04_RS24525	8787..9137	+	351	WP_000312755.1	hypothetical protein	-
A9Z04_RS24530	9777..10292	+	516	WP_001025397.1	J domain-containing protein	-
A9Z04_RS24535	10397..10939	+	543	WP_001083906.1	hypothetical protein	-
A9Z04_RS24540	10936..11187	+	252	WP_001230707.1	hypothetical protein	-
A9Z04_RS24545	11174..11401	+	228	WP_000801440.1	hypothetical protein	-
A9Z04_RS27040	11855..12154	+	300	WP_000650311.1	hypothetical protein	-
A9Z04_RS24555	12246..12596	+	351	WP_000854261.1	hypothetical protein	-
A9Z04_RS24560	12902..13150	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
A9Z04_RS24565	13147..13494	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A9Z04_RS24570	13858..14361	+	504	WP_000781818.1	hypothetical protein	-
A9Z04_RS24575	14365..15594	+	1230	WP_000539545.1	relaxase/mobilization nuclease domain-containing protein	-
A9Z04_RS24580	15671..15937	+	267	WP_000160396.1	hypothetical protein	-
A9Z04_RS24585	15942..16175	+	234	WP_000121162.1	EexN family lipoprotein	-
A9Z04_RS24590	16614..17234	+	621	WP_000157548.1	lytic transglycosylase domain-containing protein	-
A9Z04_RS24595	17302..17574	+	273	WP_001328551.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..40158	40158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T64114 WP_000520549.1 NZ_CP016008:12902-13150 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T64114 NZ_CP016008:12902-13150 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT64114 WP_000681613.1 NZ_CP016008:13147-13494 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT64114 NZ_CP016008:13147-13494 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGACTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1