64113

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 10936..11401 Replicon plasmid pNGF1_pCROD2_like
Accession NZ_CP016008
Organism Escherichia coli strain NGF1

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag A9Z04_RS24540 Protein ID WP_001230707.1
Coordinates 10936..11187 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag A9Z04_RS24545 Protein ID WP_000801440.1
Coordinates 11174..11401 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9Z04_RS24515 8241..8492 + 252 WP_000121743.1 plasmid stabilization protein -
A9Z04_RS24520 8482..8763 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin -
A9Z04_RS24525 8787..9137 + 351 WP_000312755.1 hypothetical protein -
A9Z04_RS24530 9777..10292 + 516 WP_001025397.1 J domain-containing protein -
A9Z04_RS24535 10397..10939 + 543 WP_001083906.1 hypothetical protein -
A9Z04_RS24540 10936..11187 + 252 WP_001230707.1 hypothetical protein Toxin
A9Z04_RS24545 11174..11401 + 228 WP_000801440.1 hypothetical protein Antitoxin
A9Z04_RS27040 11855..12154 + 300 WP_000650311.1 hypothetical protein -
A9Z04_RS24555 12246..12596 + 351 WP_000854261.1 hypothetical protein -
A9Z04_RS24560 12902..13150 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
A9Z04_RS24565 13147..13494 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
A9Z04_RS24570 13858..14361 + 504 WP_000781818.1 hypothetical protein -
A9Z04_RS24575 14365..15594 + 1230 WP_000539545.1 relaxase/mobilization nuclease domain-containing protein -
A9Z04_RS24580 15671..15937 + 267 WP_000160396.1 hypothetical protein -
A9Z04_RS24585 15942..16175 + 234 WP_000121162.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40158 40158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type I toxin-antitoxin system, toxin Ldr

(1-35)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T64113 WP_001230707.1 NZ_CP016008:10936-11187 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T64113 NZ_CP016008:10936-11187 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT64113 WP_000801440.1 NZ_CP016008:11174-11401 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT64113 NZ_CP016008:11174-11401 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAGAAAGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAGGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References