Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 8241..8763 Replicon plasmid pNGF1_pCROD2_like
Accession NZ_CP016008
Organism Escherichia coli strain NGF1

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag A9Z04_RS24520 Protein ID WP_000220561.1
Coordinates 8482..8763 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag A9Z04_RS24515 Protein ID WP_000121743.1
Coordinates 8241..8492 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9Z04_RS24485 3573..3875 + 303 WP_064640979.1 hypothetical protein -
A9Z04_RS24490 3912..4163 + 252 WP_000905714.1 hypothetical protein -
A9Z04_RS24500 4735..5262 - 528 WP_000577047.1 DUF2726 domain-containing protein -
A9Z04_RS24505 5398..5802 - 405 WP_000654312.1 hypothetical protein -
A9Z04_RS24510 5868..6875 - 1008 WP_000243325.1 replication initiation protein -
A9Z04_RS24515 8241..8492 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
A9Z04_RS24520 8482..8763 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A9Z04_RS24525 8787..9137 + 351 WP_000312755.1 hypothetical protein -
A9Z04_RS24530 9777..10292 + 516 WP_001025397.1 J domain-containing protein -
A9Z04_RS24535 10397..10939 + 543 WP_001083906.1 hypothetical protein -
A9Z04_RS24540 10936..11187 + 252 WP_001230707.1 hypothetical protein -
A9Z04_RS24545 11174..11401 + 228 WP_000801440.1 hypothetical protein -
A9Z04_RS27040 11855..12154 + 300 WP_000650311.1 hypothetical protein -
A9Z04_RS24555 12246..12596 + 351 WP_000854261.1 hypothetical protein -
A9Z04_RS24560 12902..13150 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
A9Z04_RS24565 13147..13494 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40158 40158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T64112 WP_000220561.1 NZ_CP016008:8482-8763 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T64112 NZ_CP016008:8482-8763 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTTGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT64112 WP_000121743.1 NZ_CP016008:8241-8492 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT64112 NZ_CP016008:8241-8492 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References