Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3904952..3905570 Replicon chromosome
Accession NZ_CP016007
Organism Escherichia coli strain NGF1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A9Z04_RS18800 Protein ID WP_001291435.1
Coordinates 3904952..3905170 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A9Z04_RS18805 Protein ID WP_000344800.1
Coordinates 3905196..3905570 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9Z04_RS18765 3900240..3900812 + 573 WP_000779831.1 YbaY family lipoprotein -
A9Z04_RS18770 3900842..3901153 - 312 WP_000409908.1 MGMT family protein -
A9Z04_RS18780 3901532..3901885 + 354 WP_000878140.1 DUF1428 domain-containing protein -
A9Z04_RS18785 3901927..3903477 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A9Z04_RS18790 3903641..3904111 - 471 WP_064640947.1 YlaC family protein -
A9Z04_RS18795 3904227..3904778 - 552 WP_000102539.1 maltose O-acetyltransferase -
A9Z04_RS18800 3904952..3905170 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A9Z04_RS18805 3905196..3905570 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A9Z04_RS18810 3906115..3909264 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
A9Z04_RS18815 3909287..3910480 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T64109 WP_001291435.1 NZ_CP016007:c3905170-3904952 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T64109 NZ_CP016007:c3905170-3904952 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT64109 WP_000344800.1 NZ_CP016007:c3905570-3905196 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT64109 NZ_CP016007:c3905570-3905196 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References