Detailed information of TA system TA064103

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 2998093..2998590
• Replicon: chromosome
• Accession: NZ_CP016007
• Organism: Escherichia coli strain NGF1

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: A9Z04_RS14440
• Protein ID: WP_001173343.1
• Coordinates: 2998417..2998590 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: A9Z04_RS14435
• Protein ID: WP_071953339.1
• Coordinates: 2998093..2998389 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9Z04_RS14415	2994821..2995540	-	720	WP_000611288.1	two-component system response regulator DcuR	-
A9Z04_RS14420	2995537..2997168	-	1632	WP_001216466.1	sensor histidine kinase	-
A9Z04_RS14425	2997349..2997579	+	231	WP_000371704.1	(4Fe-4S)-binding protein	-
A9Z04_RS14430	2997591..2997863	+	273	WP_000405647.1	N-acetyltransferase	-
A9Z04_RS14435	2998093..2998389	+	297	WP_071953339.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
A9Z04_RS14440	2998417..2998590	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
A9Z04_RS14445	2998709..3000226	-	1518	WP_000003805.1	lysine--tRNA ligase	-
A9Z04_RS14450	3000463..3001920	-	1458	WP_000856825.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T64103 WP_001173343.1 NZ_CP016007:2998417-2998590 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T64103 NZ_CP016007:2998417-2998590 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11426.92 Da
• Isoelectric Point: 4.1705

>AT64103 WP_071953339.1 NZ_CP016007:2998093-2998389 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAVTTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT64103 NZ_CP016007:2998093-2998389 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAGTAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41