Detailed information of TA system TA064087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 37806..38407
• Replicon: plasmid pS51_2
• Accession: NZ_CP015997
• Organism: Escherichia coli strain S51

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9ZW91
• Locus tag: A9K64_RS25750
• Protein ID: WP_001216033.1
• Coordinates: 37806..38186 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A2A2XEG6
• Locus tag: A9K64_RS25755
• Protein ID: WP_001697569.1
• Coordinates: 38186..38407 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K64_RS25725	33247..34731	-	1485	WP_000124155.1	hypothetical protein	-
A9K64_RS25730	34731..35924	-	1194	WP_064638618.1	hypothetical protein	-
A9K64_RS25735	36010..36462	-	453	WP_001326849.1	late promoter-activating protein	-
A9K64_RS25740	36551..37594	-	1044	WP_023356283.1	DUF968 domain-containing protein	-
A9K64_RS25745	37622..37801	-	180	WP_000113019.1	hypothetical protein	-
A9K64_RS25750	37806..38186	-	381	WP_001216033.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
A9K64_RS25755	38186..38407	-	222	WP_001697569.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A9K64_RS25760	38552..39016	-	465	WP_001697568.1	hypothetical protein	-
A9K64_RS25765	39101..39760	-	660	WP_064638621.1	hypothetical protein	-
A9K64_RS25770	39742..40116	-	375	WP_000988656.1	hypothetical protein	-
A9K64_RS25775	40123..40416	-	294	WP_000269004.1	hypothetical protein	-
A9K64_RS25780	40595..40828	-	234	WP_064638623.1	hypothetical protein	-
A9K64_RS25785	40911..41753	-	843	WP_064638625.1	DUF551 domain-containing protein	-
A9K64_RS25790	41750..41959	-	210	WP_000951712.1	hypothetical protein	-
A9K64_RS25795	41961..42686	-	726	WP_064638628.1	ead/Ea22-like family protein	-
A9K64_RS25800	42683..42922	-	240	WP_064580158.1	hypothetical protein	-
A9K64_RS25805	42915..43118	-	204	WP_000158003.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98216	98216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13571.31 Da
• Isoelectric Point: 5.6343

>T64087 WP_001216033.1 NZ_CP015997:c38186-37806 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T64087 NZ_CP015997:c38186-37806 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8163.13 Da
• Isoelectric Point: 4.7708

>AT64087 WP_001697569.1 NZ_CP015997:c38407-38186 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFETYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT64087 NZ_CP015997:c38407-38186 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTAGCGAAGTGCTCAATAATGTTGAAGCAGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAACCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: U9ZW91

Antitoxin

• AlphaFold DB: A0A2A2XEG6