Detailed information of TA system TA064086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 75476..76023
• Replicon: plasmid pS51_1
• Accession: NZ_CP015996
• Organism: Escherichia coli strain S51

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: A9K64_RS25475
• Protein ID: WP_000969983.1
• Coordinates: 75476..75757 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q5JBQ7
• Locus tag: A9K64_RS25480
• Protein ID: WP_000079943.1
• Coordinates: 75754..76023 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K64_RS25445	72066..72359	-	294	Protein_83	helix-turn-helix domain-containing protein	-
A9K64_RS25450	72414..73118	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
A9K64_RS25460	73172..73375	+	204	Protein_85	helix-turn-helix domain-containing protein	-
A9K64_RS25465	73558..74418	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
A9K64_RS25470	74582..75430	-	849	WP_001058012.1	3'-5' exonuclease	-
A9K64_RS25475	75476..75757	-	282	WP_000969983.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9K64_RS25480	75754..76023	-	270	WP_000079943.1	hypothetical protein	Antitoxin
A9K64_RS25490	76931..77962	-	1032	WP_000907875.1	replication initiation protein	-
A9K64_RS25500	78935..79198	+	264	WP_001324596.1	ash family protein	-
A9K64_RS25505	79116..79403	+	288	WP_000074849.1	conjugal transfer protein TraA	-
A9K64_RS25510	79845..80378	+	534	WP_001303319.1	transcription termination factor NusG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1A	-	1..93029	93029

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10874.52 Da
• Isoelectric Point: 7.3987

>T64086 WP_000969983.1 NZ_CP015996:c75757-75476 [Escherichia coli]
MMEIFWTMLASQDRKHIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T64086 NZ_CP015996:c75757-75476 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCATATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10272.57 Da
• Isoelectric Point: 7.2057

>AT64086 WP_000079943.1 NZ_CP015996:c76023-75754 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLSHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT64086 NZ_CP015996:c76023-75754 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGCCAGCAACTTGAGCGCGGTGATGTGCTTTCCCATGACATGGTCGAATCCTCAGCTACCGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A622US83