Detailed information of TA system TA064077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4207350..4208164
• Replicon: chromosome
• Accession: NZ_CP015995
• Organism: Escherichia coli strain S51

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: A9K64_RS21200
• Protein ID: WP_001054376.1
• Coordinates: 4207350..4207607 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: A9K64_RS21205
• Protein ID: WP_001309181.1
• Coordinates: 4207619..4208164 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K64_RS21175	4202638..4203744	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
A9K64_RS21180	4203809..4204789	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
A9K64_RS21185	4205372..4206612	-	1241	Protein_4033	helicase YjhR	-
A9K64_RS29225	4206728..4206973	+	246	Protein_4034	GNAT family N-acetyltransferase	-
A9K64_RS21200	4207350..4207607	+	258	WP_001054376.1	hypothetical protein	Toxin
A9K64_RS21205	4207619..4208164	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
A9K64_RS21210	4208220..4208966	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
A9K64_RS28560	4209135..4209353	+	219	Protein_4038	hypothetical protein	-
A9K64_RS29700	4209391..4209507	+	117	Protein_4039	VOC family protein	-
A9K64_RS21215	4209752..4210873	+	1122	WP_000010829.1	M42 family metallopeptidase	-
A9K64_RS21220	4210870..4211148	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
A9K64_RS21225	4211160..4212473	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4197138..4216388	19250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T64077 WP_001054376.1 NZ_CP015995:4207350-4207607 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T64077 NZ_CP015995:4207350-4207607 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT64077 WP_001309181.1 NZ_CP015995:4207619-4208164 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT64077 NZ_CP015995:4207619-4208164 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8