Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4160516..4161092 Replicon chromosome
Accession NZ_CP015995
Organism Escherichia coli strain S51

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag A9K64_RS20955 Protein ID WP_001295743.1
Coordinates 4160805..4161092 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A085P6S1
Locus tag A9K64_RS20950 Protein ID WP_001774708.1
Coordinates 4160516..4160818 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9K64_RS20925 4156207..4156719 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
A9K64_RS20935 4157112..4159262 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
A9K64_RS20940 4159312..4159515 + 204 WP_000467859.1 YbdD/YjiX family protein -
A9K64_RS20945 4159526..4160482 + 957 WP_001774709.1 GTPase -
A9K64_RS20950 4160516..4160818 - 303 WP_001774708.1 BrnA antitoxin family protein Antitoxin
A9K64_RS20955 4160805..4161092 - 288 WP_001295743.1 BrnT family toxin Toxin
A9K64_RS20960 4161289..4162455 + 1167 WP_000800829.1 restriction endonuclease -
A9K64_RS20965 4162519..4164138 + 1620 WP_001774706.1 type I restriction-modification system subunit M -
A9K64_RS20970 4164128..4165525 + 1398 WP_001774705.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T64076 WP_001295743.1 NZ_CP015995:c4161092-4160805 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T64076 NZ_CP015995:c4161092-4160805 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11502.00 Da        Isoelectric Point: 9.4646

>AT64076 WP_001774708.1 NZ_CP015995:c4160818-4160516 [Escherichia coli]
MSMVKHKRGAPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT64076 NZ_CP015995:c4160818-4160516 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGGCACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A085P6S1

References