Detailed information of TA system TA064062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2242315..2242953
• Replicon: chromosome
• Accession: NZ_CP015995
• Organism: Escherichia coli strain S51

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: A9K64_RS11305
• Protein ID: WP_000813794.1
• Coordinates: 2242315..2242491 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A9K64_RS11310
• Protein ID: WP_001270286.1
• Coordinates: 2242537..2242953 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K64_RS11285	2237934..2239148	-	1215	WP_001349372.1	BenE family transporter YdcO	-
A9K64_RS11290	2239201..2239737	+	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
A9K64_RS11295	2239810..2241771	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
A9K64_RS11300	2241863..2242093	-	231	WP_000494244.1	YncJ family protein	-
A9K64_RS11305	2242315..2242491	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
A9K64_RS11310	2242537..2242953	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
A9K64_RS11315	2243032..2244438	+	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
A9K64_RS11320	2244683..2245828	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
A9K64_RS11325	2245846..2246859	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
A9K64_RS11330	2246860..2247801	+	942	WP_001251319.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2236629..2237894	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T64062 WP_000813794.1 NZ_CP015995:2242315-2242491 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T64062 NZ_CP015995:2242315-2242491 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT64062 WP_001270286.1 NZ_CP015995:2242537-2242953 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT64062 NZ_CP015995:2242537-2242953 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB