Detailed information of TA system TA064054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1319196..1319821
• Replicon: chromosome
• Accession: NZ_CP015995
• Organism: Escherichia coli strain S51

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A9K64_RS06555
• Protein ID: WP_000911330.1
• Coordinates: 1319423..1319821 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A9K64_RS06550
• Protein ID: WP_000450524.1
• Coordinates: 1319196..1319423 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K64_RS06520	1314999..1315469	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
A9K64_RS06525	1315469..1316041	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
A9K64_RS06530	1316187..1317065	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A9K64_RS06535	1317082..1318116	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
A9K64_RS06545	1318329..1319042	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A9K64_RS06550	1319196..1319423	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A9K64_RS06555	1319423..1319821	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A9K64_RS06560	1319968..1320831	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
A9K64_RS06565	1320846..1322861	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
A9K64_RS06570	1322935..1323633	+	699	WP_000679823.1	esterase	-
A9K64_RS06575	1323743..1323943	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T64054 WP_000911330.1 NZ_CP015995:1319423-1319821 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T64054 NZ_CP015995:1319423-1319821 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT64054 WP_000450524.1 NZ_CP015995:1319196-1319423 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT64054 NZ_CP015995:1319196-1319423 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33