Detailed information of TA system TA064049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5425294..5425916
• Replicon: chromosome
• Accession: NZ_CP015992
• Organism: Pseudomonas sp. TCU-HL1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: THL1_RS24910
• Protein ID: WP_069085740.1
• Coordinates: 5425734..5425916 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1B3EDE3
• Locus tag: THL1_RS24905
• Protein ID: WP_069085739.1
• Coordinates: 5425294..5425701 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THL1_RS24885	5421991..5422827	+	837	WP_069085735.1	arylamine N-acetyltransferase	-
THL1_RS24890	5422968..5423723	+	756	WP_069085736.1	phytanoyl-CoA dioxygenase family protein	-
THL1_RS24895	5424220..5424690	+	471	WP_069085737.1	LEA type 2 family protein	-
THL1_RS24900	5424862..5425203	+	342	WP_069085738.1	alkylphosphonate utilization protein	-
THL1_RS24905	5425294..5425701	-	408	WP_069085739.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
THL1_RS24910	5425734..5425916	-	183	WP_069085740.1	type II toxin-antitoxin system HicA family toxin	Toxin
THL1_RS24915	5426049..5426843	+	795	WP_069085741.1	NYN domain-containing protein	-
THL1_RS24920	5427032..5428321	+	1290	WP_069085742.1	FAD-binding oxidoreductase	-
THL1_RS24925	5428519..5428779	+	261	WP_069086627.1	DUF4332 domain-containing protein	-
THL1_RS24930	5428837..5430333	-	1497	WP_069085743.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
THL1_RS24935	5430427..5430714	-	288	WP_069085744.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6773.96 Da
• Isoelectric Point: 11.3688

>T64049 WP_069085740.1 NZ_CP015992:c5425916-5425734 [Pseudomonas sp. TCU-HL1]
VNSRQLMELIEADGWFLVRTRGSHHHFKHPTKPGLVTIPHPKKDLLPRTAASILKQAGLK

Nucleotide

• Length: 183 bp

>T64049 NZ_CP015992:c5425916-5425734 [Pseudomonas sp. TCU-HL1]
GTGAATAGCCGACAACTCATGGAGCTGATCGAGGCCGATGGATGGTTTCTGGTCAGGACTCGGGGCAGTCACCACCACTT
CAAACACCCGACGAAGCCGGGCCTGGTGACGATTCCCCACCCGAAGAAGGACCTGCTGCCGCGAACGGCGGCGAGCATTC
TCAAACAGGCCGGACTCAAGTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14700.74 Da
• Isoelectric Point: 4.4085

>AT64049 WP_069085739.1 NZ_CP015992:c5425701-5425294 [Pseudomonas sp. TCU-HL1]
MLYPIAILPGDDGHAWGVEVPDLPGCFSAGDDLDDAMAMAREAIEGHLEILAEEGAPIPVARKVTFHAANPEYAGCTWAL
VDIDVTRYMGKAEKLNITLPGYLLNRIDDYVRNHPEQKSRSGFLAAAALRVLQEP

Nucleotide

• Length: 408 bp

>AT64049 NZ_CP015992:c5425701-5425294 [Pseudomonas sp. TCU-HL1]
ATGCTTTACCCAATCGCGATTCTTCCCGGTGACGATGGGCACGCCTGGGGAGTGGAGGTGCCGGACCTGCCCGGCTGCTT
CTCTGCTGGCGATGATCTGGACGACGCGATGGCCATGGCGCGCGAGGCCATCGAGGGGCATCTGGAAATTCTGGCGGAGG
AGGGCGCGCCGATTCCGGTGGCGCGGAAGGTGACCTTCCATGCGGCGAATCCGGAGTACGCCGGCTGCACCTGGGCACTG
GTGGATATAGACGTGACCCGCTACATGGGCAAGGCGGAGAAACTGAACATCACGCTACCGGGCTATCTGCTCAATCGCAT
CGATGATTACGTGAGGAACCACCCGGAGCAGAAGAGCCGTTCGGGCTTTCTGGCGGCTGCGGCGTTGCGGGTATTGCAGG
AGCCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B3EDE3