Detailed information of TA system TA064048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 3927505..3928088
• Replicon: chromosome
• Accession: NZ_CP015992
• Organism: Pseudomonas sp. TCU-HL1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: THL1_RS18215
• Protein ID: WP_069084533.1
• Coordinates: 3927708..3928088 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1B3E9L2
• Locus tag: THL1_RS18210
• Protein ID: WP_069084532.1
• Coordinates: 3927505..3927705 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THL1_RS18190	3922792..3923514	-	723	WP_069084528.1	GntR family transcriptional regulator	-
THL1_RS18195	3923818..3924354	+	537	WP_069084529.1	ATP-dependent zinc protease	-
THL1_RS18200	3924360..3925886	+	1527	WP_069084530.1	inactive transglutaminase family protein	-
THL1_RS18205	3925890..3926876	+	987	WP_069084531.1	alpha-L-glutamate ligase-like protein	-
THL1_RS18210	3927505..3927705	+	201	WP_069084532.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
THL1_RS18215	3927708..3928088	+	381	WP_069084533.1	type II toxin-antitoxin system VapC family toxin	Toxin
THL1_RS18220	3928264..3929124	-	861	WP_069084534.1	polyamine aminopropyltransferase	-
THL1_RS18225	3929337..3930197	+	861	WP_069086560.1	DNA-3-methyladenine glycosylase	-
THL1_RS18230	3930200..3930700	+	501	WP_069084535.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
THL1_RS18235	3930705..3931262	-	558	WP_069084536.1	histidine phosphatase family protein	-
THL1_RS18240	3931445..3932128	-	684	WP_069084537.1	acireductone synthase	-
THL1_RS18245	3932165..3932710	-	546	WP_069084538.1	acireductone dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13710.94 Da
• Isoelectric Point: 5.0233

>T64048 WP_069084533.1 NZ_CP015992:3927708-3928088 [Pseudomonas sp. TCU-HL1]
MAVLIDTSVWIDHFRNGNDGLVNLIGLDLAMTHPMVIVEISCGTPPAPRAQTLHNISLLLQCSQASLSEVMDFIEREKLY
GLGCGLVDIALLASTLMTPGTELWTFDKRLAELASRFGVAHNPALH

Nucleotide

• Length: 381 bp

>T64048 NZ_CP015992:3927708-3928088 [Pseudomonas sp. TCU-HL1]
ATGGCCGTGCTTATCGACACTTCGGTTTGGATAGACCACTTCCGAAACGGCAATGATGGGCTGGTTAATCTGATTGGGCT
TGATCTTGCGATGACGCATCCAATGGTTATTGTTGAAATTTCCTGCGGAACACCGCCAGCGCCAAGAGCCCAGACGCTGC
ATAACATTTCTCTTTTGCTGCAATGCAGTCAGGCGAGTTTGAGCGAGGTGATGGACTTCATCGAACGCGAAAAACTGTAT
GGCCTTGGGTGCGGGTTAGTGGACATCGCACTTCTTGCTTCCACGCTCATGACACCTGGCACAGAACTATGGACGTTTGA
TAAGCGTCTTGCTGAATTGGCCAGTAGGTTCGGCGTGGCGCACAATCCAGCCCTGCACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7364.55 Da
• Isoelectric Point: 9.9929

>AT64048 WP_069084532.1 NZ_CP015992:3927505-3927705 [Pseudomonas sp. TCU-HL1]
MRTTVTIDDALYQKALEMADPGMDKADIFREAIKTFVRVQAGKRLAALGGTMPEIRDVPRRRGESV

Nucleotide

• Length: 201 bp

>AT64048 NZ_CP015992:3927505-3927705 [Pseudomonas sp. TCU-HL1]
ATGCGCACCACTGTCACCATTGATGACGCTCTCTACCAAAAGGCGCTTGAAATGGCCGATCCGGGCATGGATAAAGCAGA
CATTTTCCGCGAAGCCATCAAAACCTTTGTACGAGTGCAAGCAGGAAAGCGTCTTGCAGCCCTTGGAGGGACGATGCCTG
AGATTCGGGACGTGCCGCGCCGCCGTGGCGAGTCAGTTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1B3E9L2