Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7444..8087 Replicon plasmid pWSZBR
Accession NZ_CP015991
Organism Klebsiella pneumoniae strain BR

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag A9J37_RS26140 Protein ID WP_000754566.1
Coordinates 7671..8087 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag A9J37_RS26135 Protein ID WP_001261276.1
Coordinates 7444..7674 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9J37_RS26105 3012..3878 - 867 WP_004118283.1 replication initiation protein -
A9J37_RS29320 4405..4509 + 105 WP_032409716.1 hypothetical protein -
A9J37_RS26115 4639..4896 + 258 WP_000764642.1 hypothetical protein -
A9J37_RS26120 4954..5730 - 777 WP_000015958.1 site-specific integrase -
A9J37_RS26125 5727..6260 - 534 WP_020326162.1 hypothetical protein -
A9J37_RS26130 6520..6870 - 351 WP_000493378.1 hypothetical protein -
A9J37_RS26135 7444..7674 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A9J37_RS26140 7671..8087 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
A9J37_RS28280 8291..9147 + 857 Protein_11 IS3-like element ISEc15 family transposase -
A9J37_RS26155 9429..10505 + 1077 WP_000227969.1 IS110 family transposase -
A9J37_RS27945 11481..12116 - 636 WP_004118217.1 His-Xaa-Ser repeat protein HxsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / qnrA7 / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / blaKPC-2 - 1..76251 76251


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T64044 WP_000754566.1 NZ_CP015991:7671-8087 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T64044 NZ_CP015991:7671-8087 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT64044 WP_001261276.1 NZ_CP015991:7444-7674 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT64044 NZ_CP015991:7444-7674 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References