64025

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/Tad-HTH_37
Location 156525..157222 Replicon plasmid pEP2
Accession NZ_CP015989
Organism Sphingobium sp. EP60837

Toxin (Protein)


Gene name tad Uniprot ID T0J3S2
Locus tag EP837_RS20130 Protein ID WP_013041565.1
Coordinates 156848..157222 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID T0G937
Locus tag EP837_RS20125 Protein ID WP_006964370.1
Coordinates 156525..156851 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EP837_RS20090 151594..152361 - 768 WP_007686288.1 IS21-like element ISSsp5 family helper ATPase IstB -
EP837_RS20095 152358..153851 - 1494 WP_011950768.1 IS21 family transposase -
EP837_RS20100 153954..154400 + 447 WP_156518815.1 hypothetical protein -
EP837_RS20105 154460..154690 + 231 WP_004213230.1 hypothetical protein -
EP837_RS20110 154826..155590 - 765 WP_006949122.1 IS6 family transposase -
EP837_RS20860 155656..155970 + 315 Protein_160 muramidase -
EP837_RS20115 155991..156290 - 300 WP_013041567.1 hypothetical protein -
EP837_RS20120 156283..156525 - 243 WP_013041566.1 hypothetical protein -
EP837_RS20125 156525..156851 - 327 WP_006964370.1 helix-turn-helix domain-containing protein Antitoxin
EP837_RS20130 156848..157222 - 375 WP_013041565.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EP837_RS21480 157995..158075 - 81 Protein_165 Tn3 family transposase -
EP837_RS21220 158062..158208 - 147 Protein_166 oxidoreductase -
EP837_RS20135 158296..159060 - 765 WP_006949122.1 IS6 family transposase -
EP837_RS20140 159127..159468 + 342 Protein_168 HlyD family secretion protein -
EP837_RS20145 159470..160993 + 1524 WP_066532663.1 DHA2 family efflux MFS transporter permease subunit -
EP837_RS20150 161003..161662 + 660 WP_020817748.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..192095 192095
- inside IScluster/Tn - - 148534..159060 10526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-108)

Antitoxin

HigA2-like, helix-turn-helix domain

(15-94)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.67 Da        Isoelectric Point: 9.4885

>T64025 WP_013041565.1 NZ_CP015989:c157222-156848 [Sphingobium sp. EP60837]
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDHQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKSAEEHHRQHQTPRGTA

Download         Length: 375 bp

>T64025 NZ_CP015989:c157222-156848 [Sphingobium sp. EP60837]
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTTGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATCATAGACGACCACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTCGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAATCCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11792.57 Da        Isoelectric Point: 7.1681

>AT64025 WP_006964370.1 NZ_CP015989:c156851-156525 [Sphingobium sp. EP60837]
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA

Download         Length: 327 bp

>AT64025 NZ_CP015989:c156851-156525 [Sphingobium sp. EP60837]
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGACCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTGGCCGAGGCCATGCAGGAGCGGAAGTTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACCCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTAGCC
ATGCTCACCCGGCTTGGCTGCAAGGTCGATATTGTCGTGCGCCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J7XHG4

Antitoxin

Source ID Structure
AlphaFold DB A0A0J8A803

References