Detailed information of TA system TA064024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 200651..201291
• Replicon: plasmid pEP1
• Accession: NZ_CP015988
• Organism: Sphingobium sp. EP60837

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A173L2V0
• Locus tag: EP837_RS18930
• Protein ID: WP_066532158.1
• Coordinates: 200881..201291 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EP837_RS20775
• Protein ID: WP_082919875.1
• Coordinates: 200651..200881 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP837_RS18920	197077..198306	-	1230	WP_082919864.1	aminodeoxychorismate synthase component I	-
EP837_RS18925	198677..199372	-	696	WP_156518775.1	alpha/beta hydrolase	-
EP837_RS20775	200651..200881	+	231	WP_082919875.1	antitoxin	Antitoxin
EP837_RS18930	200881..201291	+	411	WP_066532158.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EP837_RS18935	201399..201644	+	246	WP_066532161.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
EP837_RS18940	201641..202030	+	390	WP_066532163.1	type II toxin-antitoxin system VapC family toxin	-
EP837_RS18945	202275..202679	+	405	WP_066532165.1	metal-binding protein	-
EP837_RS18950	202823..204160	+	1338	WP_066532167.1	dGTPase	-
EP837_RS18955	204631..205113	+	483	WP_066532169.1	Lrp/AsnC family transcriptional regulator	-
EP837_RS18960	205119..205676	+	558	WP_197486427.1	Lrp/AsnC family transcriptional regulator	-
EP837_RS18965	205829..206065	+	237	WP_066532173.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..269215	269215

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15123.21 Da
• Isoelectric Point: 6.7132

>T64024 WP_066532158.1 NZ_CP015988:200881-201291 [Sphingobium sp. EP60837]
MLKFMLDTNICIFTIKNRPQEVRSAFKQHQGQMCISSVTLMELIYGAEKSSNPERNLADVEGFAARLEVLNYGREAAAHT
GQLRAELSRAGTPIGPYDQMIAGHARSAGLIIVTNNQREFERVPGLRVEDWVASSD

Nucleotide

• Length: 411 bp

>T64024 NZ_CP015988:200881-201291 [Sphingobium sp. EP60837]
ATGCTGAAGTTCATGCTGGATACCAACATCTGCATTTTCACTATCAAGAACCGGCCTCAGGAGGTGCGGAGCGCTTTTAA
GCAGCATCAGGGCCAAATGTGCATCAGTTCTGTCACGCTGATGGAGTTGATTTATGGCGCGGAAAAATCCTCTAATCCAG
AGCGCAATTTGGCAGACGTGGAGGGCTTTGCGGCGCGTTTGGAGGTGCTCAACTATGGCCGGGAAGCAGCTGCGCACACT
GGACAGCTTCGTGCCGAACTTTCGCGAGCTGGGACGCCTATAGGCCCCTATGACCAGATGATTGCGGGACATGCTCGATC
GGCGGGTTTAATAATTGTCACAAATAATCAGCGGGAATTCGAGCGCGTCCCGGGACTTCGAGTTGAAGACTGGGTCGCGA
GTTCCGATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8462.37 Da
• Isoelectric Point: 4.3867

>AT64024 WP_082919875.1 NZ_CP015988:200651-200881 [Sphingobium sp. EP60837]
VEQGSVFQTNRSQAVRLPKAAALPDDVKRVDVIALGRTRIITPAGEGWDSWFEGEGVTTDFMSEREQPADQTREPF

Nucleotide

• Length: 231 bp

>AT64024 NZ_CP015988:200651-200881 [Sphingobium sp. EP60837]
GTGGAACAAGGTTCTGTATTTCAGACCAACAGGAGCCAAGCAGTGCGTTTGCCAAAGGCTGCCGCCCTCCCCGACGACGT
GAAGCGAGTTGATGTTATAGCCTTAGGTCGAACGCGTATCATCACCCCTGCAGGTGAGGGTTGGGACAGCTGGTTTGAAG
GCGAAGGTGTCACTACTGACTTCATGTCAGAACGCGAGCAGCCGGCAGATCAGACCCGCGAGCCCTTCTGA

Structures

Toxin

• AlphaFold DB: A0A173L2V0