Detailed information of TA system TA064023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 173615..174282
• Replicon: chromosome
• Accession: NZ_CP015987
• Organism: Sphingobium sp. EP60837

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A173KYY5
• Locus tag: EP837_RS13935
• Protein ID: WP_066529804.1
• Coordinates: 173863..174282 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EP837_RS20535
• Protein ID: WP_082919701.1
• Coordinates: 173615..173863 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP837_RS13925	169314..173063	+	3750	WP_066529800.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
EP837_RS13930	173060..173473	-	414	WP_066529802.1	hypothetical protein	-
EP837_RS20535	173615..173863	+	249	WP_082919701.1	Arc family DNA-binding protein	Antitoxin
EP837_RS13935	173863..174282	+	420	WP_066529804.1	type II toxin-antitoxin system VapC family toxin	Toxin
EP837_RS13940	174294..175211	+	918	WP_066529813.1	HEPN domain-containing protein	-
EP837_RS21370	175669..176310	+	642	WP_066529815.1	hypothetical protein	-
EP837_RS13950	176313..177527	+	1215	WP_066529822.1	phosphotransferase	-
EP837_RS13955	177524..178564	+	1041	WP_066529823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-127)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14964.35 Da
• Isoelectric Point: 5.8373

>T64023 WP_066529804.1 NZ_CP015987:173863-174282 [Sphingobium sp. EP60837]
MIVLDTNVVLEAMKPSAEPVVVDWLNRQPAETLFLTSVTLAELLFGITALTEGKRKNALASALDRLLKLFGERILPFETN
AAQSYAALAFHAQAVGKYFPTPDGYIAAIAKATGFSVATRDTAPFDAVGIAVINPWISR

Nucleotide

• Length: 420 bp

>T64023 NZ_CP015987:173863-174282 [Sphingobium sp. EP60837]
ATGATCGTCCTGGATACCAACGTTGTTTTGGAGGCCATGAAGCCGTCAGCGGAGCCGGTGGTCGTCGACTGGCTTAATCG
CCAGCCGGCCGAGACACTCTTCCTGACCAGCGTGACGCTCGCTGAACTGTTGTTCGGTATTACAGCCCTAACCGAAGGCA
AGCGCAAAAATGCCCTCGCCTCTGCATTGGACAGGCTCTTGAAACTTTTTGGCGAGCGAATCCTCCCGTTCGAGACCAAC
GCTGCGCAAAGCTACGCCGCCCTCGCCTTCCACGCCCAGGCTGTCGGCAAATATTTTCCAACTCCAGACGGCTACATCGC
CGCGATTGCAAAGGCGACCGGCTTCTCAGTGGCGACACGTGATACCGCTCCCTTCGACGCGGTTGGCATTGCCGTAATCA
ACCCCTGGATTTCGCGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8918.11 Da
• Isoelectric Point: 9.0167

>AT64023 WP_082919701.1 NZ_CP015987:173615-173863 [Sphingobium sp. EP60837]
VPSVTVRNLPDEVHRAIRARAASHGRSTEAEIRDILEQAVKPAGRLRMGDALAQIGRQAGLTDADGDWLAQKRMPTAPMS
FD

Nucleotide

• Length: 249 bp

>AT64023 NZ_CP015987:173615-173863 [Sphingobium sp. EP60837]
GTGCCATCGGTGACTGTGAGGAATTTGCCAGATGAGGTTCATCGGGCAATCCGCGCGCGTGCAGCCTCCCATGGAAGAAG
CACGGAAGCGGAAATTCGCGACATTTTGGAGCAAGCAGTAAAGCCGGCCGGCCGGCTTCGCATGGGCGATGCTCTTGCCC
AGATCGGCAGGCAAGCGGGCCTGACGGATGCTGATGGAGATTGGCTCGCCCAGAAGCGTATGCCCACCGCCCCCATGAGC
TTTGATTGA

Structures

Toxin

• AlphaFold DB: A0A173KYY5