Detailed information of TA system TA064021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 42020..42597
• Replicon: plasmid pl2015TE24968
• Accession: NZ_CP015985
• Organism: Listeria monocytogenes strain 2015TE24968

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: AXF54_RS14730
• Protein ID: WP_003728465.1
• Coordinates: 42256..42597 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: AXF54_RS14725
• Protein ID: WP_003728464.1
• Coordinates: 42020..42262 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXF54_RS14680	37292..38008	-	717	WP_003731678.1	AP2 domain-containing protein	-
AXF54_RS14685	38024..38746	-	723	WP_003728517.1	CRISPR-associated protein Cas5	-
AXF54_RS14690	38750..39175	-	426	WP_003728518.1	hypothetical protein	-
AXF54_RS14695	39176..39424	-	249	WP_003728519.1	hypothetical protein	-
AXF54_RS14700	39546..40034	-	489	WP_003728520.1	hypothetical protein	-
AXF54_RS14705	40193..40570	-	378	WP_188317290.1	DUF3850 domain-containing protein	-
AXF54_RS14710	40589..41251	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
AXF54_RS14715	41251..41577	-	327	WP_003728462.1	hypothetical protein	-
AXF54_RS14720	41708..41959	+	252	WP_003728463.1	hypothetical protein	-
AXF54_RS14725	42020..42262	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AXF54_RS14730	42256..42597	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AXF54_RS14735	42790..44925	-	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
AXF54_RS14740	44925..45284	-	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
AXF54_RS14745	45564..46118	+	555	WP_003728468.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57530	57530

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-78)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 14629.86 Da
• Isoelectric Point: 12.8531

>T64021 WP_003728465.1 NZ_CP015985:42256-42597 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 342 bp

>T64021 NZ_CP015985:42256-42597 [Listeria monocytogenes]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 10400.12 Da
• Isoelectric Point: 12.7030

>AT64021 WP_003728464.1 NZ_CP015985:42020-42262 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
K

Nucleotide

• Length: 243 bp

>AT64021 NZ_CP015985:42020-42262 [Listeria monocytogenes]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5