Detailed information of TA system TA064016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 500624..501260
• Replicon: chromosome
• Accession: NZ_CP015975
• Organism: Bacillus subtilis subsp. subtilis strain delta6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: A8O17_RS02570
• Protein ID: WP_003156187.1
• Coordinates: 500910..501260 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: A8O17_RS02565
• Protein ID: WP_003225183.1
• Coordinates: 500624..500905 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8O17_RS02545	496983..497582	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
A8O17_RS02550	497677..498042	+	366	WP_003234281.1	holo-ACP synthase	-
A8O17_RS02555	498208..499224	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
A8O17_RS02560	499339..500508	+	1170	WP_003234284.1	alanine racemase	-
A8O17_RS02565	500624..500905	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
A8O17_RS02570	500910..501260	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
A8O17_RS02575	501375..502199	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
A8O17_RS02580	502204..502569	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
A8O17_RS02585	502573..502974	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
A8O17_RS02590	502986..503993	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
A8O17_RS02595	504055..504384	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
A8O17_RS02600	504381..504863	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
A8O17_RS02605	504829..505617	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
A8O17_RS02610	505617..506216	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T64016 WP_003156187.1 NZ_CP015975:500910-501260 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T64016 NZ_CP015975:500910-501260 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT64016 WP_003225183.1 NZ_CP015975:500624-500905 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT64016 NZ_CP015975:500624-500905 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32