Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 11820..12409 Replicon plasmid pXAG27.12-2
Accession NZ_CP015973
Organism Xanthomonas citri pv. glycines str. 12-2

Toxin (Protein)


Gene name pemK Uniprot ID Q32X98
Locus tag A9D66_RS24485 Protein ID WP_012477455.1
Coordinates 12077..12409 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q32X99
Locus tag A9D66_RS24480 Protein ID WP_012477454.1
Coordinates 11820..12077 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9D66_RS24450 7545..8465 - 921 WP_012477431.1 RepA -
A9D66_RS24455 8531..9337 - 807 WP_016851361.1 serine protease -
A9D66_RS25460 9334..9492 - 159 WP_154666355.1 hypothetical protein -
A9D66_RS24460 9715..9969 - 255 WP_012477434.1 hypothetical protein -
A9D66_RS24465 9969..10619 - 651 WP_012477435.1 ParA family protein -
A9D66_RS24470 10686..11330 - 645 WP_012477436.1 recombinase family protein -
A9D66_RS24475 11327..11554 - 228 WP_012477437.1 hypothetical protein -
A9D66_RS24480 11820..12077 + 258 WP_012477454.1 plasmid stable inheritance protein I Antitoxin
A9D66_RS24485 12077..12409 + 333 WP_012477455.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A9D66_RS24490 12498..13316 - 819 WP_012477456.1 hypothetical protein -
A9D66_RS24495 13344..15521 - 2178 WP_012477457.1 relaxase/mobilization nuclease domain-containing protein -
A9D66_RS24500 15523..15825 - 303 WP_012477458.1 hypothetical protein -
A9D66_RS25200 16113..16532 + 420 WP_012477459.1 hypothetical protein -
A9D66_RS24505 16522..17226 + 705 WP_007975110.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..26618 26618


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11581.24 Da        Isoelectric Point: 9.1684

>T64015 WP_012477455.1 NZ_CP015973:12077-12409 [Xanthomonas citri pv. glycines str. 12-2]
MERGDIYLVSLDPTSGHEQQGTRPVLIVSPGPFNRLTKTPVVLPITTGGNFARTAGFTVSLTGGGTKTTGVVRCDQPRAL
DIGSRNGRKLESVSAAITDEVMAKLATILE

Download         Length: 333 bp

>T64015 NZ_CP015973:12077-12409 [Xanthomonas citri pv. glycines str. 12-2]
ATGGAGCGGGGTGATATCTATCTTGTGTCTCTCGATCCTACTTCCGGCCATGAGCAACAGGGCACTCGCCCGGTCCTGAT
TGTGTCGCCGGGACCGTTCAACCGCCTTACCAAAACACCCGTGGTGCTACCGATCACCACGGGTGGCAATTTCGCCCGTA
CGGCAGGTTTCACGGTATCCCTCACGGGGGGAGGCACCAAAACGACCGGAGTCGTGCGCTGCGATCAACCGCGCGCCCTG
GACATTGGATCGCGTAACGGCCGCAAGCTGGAAAGCGTCTCAGCAGCGATCACCGATGAGGTGATGGCTAAGCTGGCGAC
CATACTTGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 8951.34 Da        Isoelectric Point: 5.5491

>AT64015 WP_012477454.1 NZ_CP015973:11820-12077 [Xanthomonas citri pv. glycines str. 12-2]
MHTTNLRKVGGSVMLAVPPALLDVLHIGAGAKVGLAVDNGRLVVEPHARPRYTLADLLAQCDASAELASEDREWLDAKPV
GGELL

Download         Length: 258 bp

>AT64015 NZ_CP015973:11820-12077 [Xanthomonas citri pv. glycines str. 12-2]
ATGCACACGACAAATCTGCGCAAGGTTGGCGGTTCGGTCATGCTCGCGGTTCCGCCTGCGCTGCTCGATGTGCTGCACAT
CGGGGCAGGGGCAAAAGTGGGCTTGGCTGTCGATAACGGCCGCTTGGTAGTCGAGCCACATGCGCGCCCGCGCTACACCC
TCGCCGACCTGCTCGCCCAATGCGATGCGTCAGCCGAGCTGGCTTCCGAAGATCGGGAATGGCTTGACGCCAAGCCTGTT
GGCGGCGAGCTGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z6Z9Y5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9EPT3

References