Detailed information of TA system TA064008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2334214..2334775
• Replicon: chromosome
• Accession: NZ_CP015970
• Organism: Acidipropionibacterium acidipropionici strain F3E8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K7S927
• Locus tag: A8L58_RS10865
• Protein ID: WP_015071954.1
• Coordinates: 2334214..2334561 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K7S105
• Locus tag: A8L58_RS10870
• Protein ID: WP_015071955.1
• Coordinates: 2334548..2334775 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L58_RS10845	2329380..2330354	+	975	WP_198401490.1	zinc-binding alcohol dehydrogenase family protein	-
A8L58_RS10850	2330485..2330862	-	378	WP_082816119.1	L,D-transpeptidase	-
A8L58_RS10855	2331392..2332936	-	1545	WP_062819698.1	glycoside hydrolase family 32 protein	-
A8L58_RS10860	2333102..2334061	+	960	WP_062819699.1	ribokinase	-
A8L58_RS10865	2334214..2334561	-	348	WP_015071954.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A8L58_RS10870	2334548..2334775	-	228	WP_015071955.1	ribbon-helix-helix domain-containing protein	Antitoxin
A8L58_RS10875	2335102..2337243	+	2142	WP_062819700.1	NAD(+) synthase	-
A8L58_RS10880	2337353..2337757	-	405	WP_015071959.1	nitrite reductase small subunit NirD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-35)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12548.53 Da
• Isoelectric Point: 11.4911

>T64008 WP_015071954.1 NZ_CP015970:c2334561-2334214 [Acidipropionibacterium acidipropionici]
MIRGEVRLVDLDPARPGEVNKRRPAVLVSNDTANRTAARLGRGVVTVVPVTSNVTRVFPFQVLLQADETGLRRDSKAQAE
QVRSVSVERIGPVLGMVPADLMSRIDDALRIHLGL

Nucleotide

• Length: 348 bp

>T64008 NZ_CP015970:c2334561-2334214 [Acidipropionibacterium acidipropionici]
ATGATCCGCGGTGAGGTCCGGCTGGTCGATCTGGATCCGGCGCGCCCCGGCGAGGTGAACAAGCGACGTCCCGCCGTCCT
CGTGAGCAATGACACGGCGAACCGGACGGCCGCACGGCTGGGGCGGGGAGTGGTGACGGTGGTGCCGGTCACCAGCAACG
TCACCCGGGTCTTCCCCTTCCAGGTCCTGCTTCAGGCGGACGAGACGGGGCTGCGGCGCGATTCCAAGGCCCAGGCCGAG
CAGGTCCGCTCGGTGTCGGTGGAACGGATCGGCCCGGTGCTCGGGATGGTGCCGGCTGATCTCATGAGCCGCATCGATGA
CGCGCTGAGAATCCACCTGGGGCTGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8161.01 Da
• Isoelectric Point: 4.0941

>AT64008 WP_015071955.1 NZ_CP015970:c2334775-2334548 [Acidipropionibacterium acidipropionici]
MKLSVSLSKEQVETIDRYIRATGMRSRSAVIQKAIGMLGAPELEGDYAAAWDEWESGDAEAWESTAADGLGDDPR

Nucleotide

• Length: 228 bp

>AT64008 NZ_CP015970:c2334775-2334548 [Acidipropionibacterium acidipropionici]
ATGAAACTCAGTGTCAGCCTGTCCAAGGAGCAGGTGGAGACCATCGACCGGTACATCCGTGCCACCGGGATGAGGTCGAG
GTCCGCGGTGATCCAGAAGGCGATCGGCATGCTCGGCGCCCCGGAGCTCGAGGGGGACTACGCCGCCGCCTGGGACGAGT
GGGAGTCCGGGGACGCCGAGGCCTGGGAGTCGACGGCCGCCGATGGGCTCGGCGATGATCCGCGGTGA

Structures

Toxin

• AlphaFold DB: K7S927

Antitoxin

• AlphaFold DB: K7S105