Detailed information of TA system TA064003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 2413447..2414439
• Replicon: chromosome
• Accession: NZ_CP015965
• Organism: Mycobacterium intracellulare subsp. yongonense strain Asan 36527

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: S4ZAV9
• Locus tag: MOTT27_RS11395
• Protein ID: WP_014711570.1
• Coordinates: 2413861..2414439 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: I2ADH5
• Locus tag: MOTT27_RS11390
• Protein ID: WP_008256419.1
• Coordinates: 2413447..2413836 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOTT27_RS11370	2408598..2409266	-	669	WP_179945108.1	hypothetical protein	-
MOTT27_RS11375	2409346..2410863	+	1518	WP_014711567.1	carboxylesterase/lipase family protein	-
MOTT27_RS11380	2410882..2412885	+	2004	WP_014711568.1	phosphotransferase	-
MOTT27_RS11385	2412882..2413376	+	495	WP_014711569.1	gluconokinase	-
MOTT27_RS11390	2413447..2413836	+	390	WP_008256419.1	hypothetical protein	Antitoxin
MOTT27_RS11395	2413861..2414439	+	579	WP_014711570.1	zeta toxin family protein	Toxin
MOTT27_RS11400	2414449..2415516	-	1068	WP_020822441.1	DoxX family membrane protein	-
MOTT27_RS11405	2415612..2416172	+	561	WP_014711572.1	pyrazinamidase PncA	-
MOTT27_RS11410	2416172..2416972	+	801	WP_020822443.1	nuclear transport factor 2 family protein	-
MOTT27_RS11415	2416984..2417334	-	351	WP_008256426.1	DoxX family protein	-
MOTT27_RS11420	2417500..2418471	+	972	WP_020822445.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-137)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21009.00 Da
• Isoelectric Point: 11.0233

>T64003 WP_014711570.1 NZ_CP015965:2413861-2414439 [Mycobacterium intracellulare subsp. yongonense]
MQRLDLVVGPNGAGKSTFIAFTLAPLLPGSPVVNADEIARQRWPGESAGRAYEAAQIAADTRAKLIDLGRSFIAETVFSH
PSKLDLIRTAHRAGFTVVLHAMLIPEDLAVERVRHRVRAGGHDVPETKVRERHRRLWTPVTDAIALSDVATVYDNSRLRG
PRIVARLTGGTTIGSPDWPAWTPGALRSRWAD

Nucleotide

• Length: 579 bp

>T64003 NZ_CP015965:2413861-2414439 [Mycobacterium intracellulare subsp. yongonense]
ATGCAGCGACTTGACCTGGTGGTCGGGCCGAACGGTGCCGGCAAGTCGACCTTCATCGCGTTCACCCTGGCGCCGCTGTT
GCCCGGCAGCCCGGTCGTCAACGCCGACGAAATCGCAAGGCAGCGCTGGCCGGGGGAGTCGGCGGGGCGCGCCTACGAGG
CCGCGCAAATCGCCGCCGACACGCGCGCGAAGTTGATCGACCTGGGGCGGTCGTTTATCGCGGAGACCGTGTTTTCCCAC
CCGTCGAAGCTGGACCTCATCCGCACCGCCCACCGAGCGGGCTTCACCGTCGTCCTGCACGCGATGCTCATCCCGGAAGA
CCTTGCCGTCGAACGTGTCCGGCACCGCGTGCGGGCCGGCGGCCACGACGTACCGGAAACCAAGGTCCGAGAGCGCCATC
GTCGCTTGTGGACGCCAGTCACCGACGCCATCGCGCTGTCCGACGTCGCCACCGTCTACGACAACAGCCGCCTTCGCGGG
CCGCGCATCGTCGCCCGCCTCACCGGCGGCACGACGATCGGTTCACCGGACTGGCCCGCGTGGACGCCAGGGGCCTTGCG
GTCACGCTGGGCGGACTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13518.97 Da
• Isoelectric Point: 5.1668

>AT64003 WP_008256419.1 NZ_CP015965:2413447-2413836 [Mycobacterium intracellulare subsp. yongonense]
MADAPDRVTRVASDLMDSAASEGARQSRSAKQQLDHWARVGRAVSSQHTASRRRVEAAMAGQLATGELTVEEGVVFNAEI
SAAIEESLVHANYGATLAGHGVTTVALNEDGDIVEHLPDGAAVMLARGR

Nucleotide

• Length: 390 bp

>AT64003 NZ_CP015965:2413447-2413836 [Mycobacterium intracellulare subsp. yongonense]
ATGGCCGACGCTCCCGATCGTGTGACCCGGGTGGCGTCCGACCTGATGGACAGCGCGGCCTCCGAAGGCGCCCGCCAGAG
CCGTTCGGCCAAGCAGCAACTCGACCACTGGGCGCGTGTCGGGCGCGCGGTGTCCAGCCAGCACACCGCCTCGCGTCGGC
GGGTCGAGGCCGCGATGGCCGGCCAGCTGGCGACCGGCGAACTCACCGTCGAGGAAGGCGTGGTGTTCAACGCCGAGATC
TCCGCGGCTATTGAAGAAAGCCTGGTGCACGCCAACTACGGGGCGACGCTGGCCGGGCATGGGGTCACCACGGTCGCCCT
CAACGAGGACGGCGACATCGTCGAGCACCTGCCCGATGGCGCCGCCGTAATGCTGGCGCGCGGGCGCTGA

Structures

Toxin

• AlphaFold DB: S4ZAV9

Antitoxin

• AlphaFold DB: I2ADH5