Detailed information of TA system TA064002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1144758..1145320
• Replicon: chromosome
• Accession: NZ_CP015965
• Organism: Mycobacterium intracellulare subsp. yongonense strain Asan 36527

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A081I0A5
• Locus tag: MOTT27_RS05755
• Protein ID: WP_036454801.1
• Coordinates: 1144952..1145320 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A081I0A4
• Locus tag: MOTT27_RS05750
• Protein ID: WP_036455532.1
• Coordinates: 1144758..1144955 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOTT27_RS05720	1140175..1140915	+	741	WP_036454796.1	aminoglycoside 3'-phosphotransferase	-
MOTT27_RS05725	1141030..1142847	+	1818	WP_162288420.1	transposase	-
MOTT27_RS05730	1143055..1143339	+	285	WP_020821851.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MOTT27_RS05735	1143354..1143599	+	246	WP_036455529.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MOTT27_RS05740	1143635..1144186	+	552	WP_036454798.1	VOC family protein	-
MOTT27_RS05745	1144265..1144663	+	399	WP_036454800.1	VOC family protein	-
MOTT27_RS05750	1144758..1144955	+	198	WP_036455532.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MOTT27_RS05755	1144952..1145320	+	369	WP_036454801.1	type II toxin-antitoxin system VapC family toxin	Toxin
MOTT27_RS05760	1145503..1145844	+	342	WP_179945069.1	hypothetical protein	-
MOTT27_RS25940	1145930..1146289	+	360	WP_042910935.1	hypothetical protein	-
MOTT27_RS05770	1146474..1147009	+	536	Protein_1125	C40 family peptidase	-
MOTT27_RS05775	1147371..1147694	+	324	WP_036454806.1	antibiotic biosynthesis monooxygenase	-
MOTT27_RS05780	1147732..1148565	-	834	WP_036454808.1	SAM-dependent methyltransferase	-
MOTT27_RS05785	1148600..1149283	-	684	WP_036455537.1	hypothetical protein	-
MOTT27_RS05790	1149430..1149639	+	210	WP_072501223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13435.74 Da
• Isoelectric Point: 6.4862

>T64002 WP_036454801.1 NZ_CP015965:1144952-1145320 [Mycobacterium intracellulare subsp. yongonense]
MILVDTAVWIDHLHTAELRLVKLLEIDQVGCHALVIEELALGSISRREVVLDLLANLRQFPTVQHAEILHLTGQRKLYGR
GLSAVDVHLMAAVALVEGAQLWTRDKRLKAASVDAGIALFDA

Nucleotide

• Length: 369 bp

>T64002 NZ_CP015965:1144952-1145320 [Mycobacterium intracellulare subsp. yongonense]
ATGATCCTCGTTGACACCGCGGTGTGGATCGATCATCTACACACCGCGGAGTTGCGGCTAGTCAAGCTGCTGGAGATCGA
CCAAGTTGGCTGTCATGCGCTGGTCATCGAAGAGCTGGCGCTGGGGTCGATCTCGCGACGTGAGGTGGTGCTCGACCTCC
TCGCGAATCTTCGCCAGTTTCCGACCGTCCAGCACGCCGAGATCCTTCACCTCACCGGTCAACGCAAACTGTATGGACGC
GGTCTCAGTGCGGTCGACGTCCATCTCATGGCCGCCGTCGCCCTGGTTGAAGGCGCGCAGCTGTGGACGAGAGACAAGCG
GTTGAAGGCTGCGTCGGTCGATGCGGGCATCGCACTCTTCGATGCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6871.88 Da
• Isoelectric Point: 11.6072

>AT64002 WP_036455532.1 NZ_CP015965:1144758-1144955 [Mycobacterium intracellulare subsp. yongonense]
MRTTVTIDDDLLAKAAELTGVRENVALLREGLQTLIRVESARRLAALGGTDAGAVAAPRRRRGTG

Nucleotide

• Length: 198 bp

>AT64002 NZ_CP015965:1144758-1144955 [Mycobacterium intracellulare subsp. yongonense]
ATGCGGACCACAGTCACGATCGACGACGACCTGTTGGCCAAGGCTGCTGAGTTGACCGGCGTGCGCGAGAACGTCGCGTT
GCTCCGCGAGGGATTGCAGACGCTGATCAGGGTGGAAAGCGCTCGCCGACTTGCCGCGCTCGGCGGGACGGATGCCGGGG
CGGTGGCCGCTCCCCGGCGCCGCCGTGGGACTGGATGA

Structures

Toxin

• AlphaFold DB: A0A081I0A5

Antitoxin

• AlphaFold DB: A0A081I0A4