Detailed information of TA system TA064000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 896686..897339
• Replicon: chromosome
• Accession: NZ_CP015965
• Organism: Mycobacterium intracellulare subsp. yongonense strain Asan 36527

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MOTT27_RS04585
• Protein ID: WP_008253911.1
• Coordinates: 896878..897339 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I2A997
• Locus tag: MOTT27_RS04580
• Protein ID: WP_008253910.1
• Coordinates: 896686..896874 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOTT27_RS04570	892659..894236	+	1578	WP_014381859.1	serine hydrolase	-
MOTT27_RS04575	894233..896620	+	2388	WP_014711030.1	cation-translocating P-type ATPase	-
MOTT27_RS04580	896686..896874	+	189	WP_008253910.1	antitoxin	Antitoxin
MOTT27_RS04585	896878..897339	+	462	WP_008253911.1	SRPBCC family protein	Toxin
MOTT27_RS04590	897424..898194	+	771	WP_008253913.1	VOC family protein	-
MOTT27_RS04595	898268..898702	+	435	WP_008253914.1	hypothetical protein	-
MOTT27_RS04600	898707..899195	-	489	WP_036454690.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
MOTT27_RS04605	899195..900709	-	1515	WP_036454693.1	carotenoid oxygenase family protein	-
MOTT27_RS04610	900712..901935	-	1224	WP_036454694.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T64000 WP_008253911.1 NZ_CP015965:896878-897339 [Mycobacterium intracellulare subsp. yongonense]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T64000 NZ_CP015965:896878-897339 [Mycobacterium intracellulare subsp. yongonense]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TTGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6797.44 Da
• Isoelectric Point: 4.4702

>AT64000 WP_008253910.1 NZ_CP015965:896686-896874 [Mycobacterium intracellulare subsp. yongonense]
MGFLDKAKDLLWQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT64000 NZ_CP015965:896686-896874 [Mycobacterium intracellulare subsp. yongonense]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTGGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7TPT2