Detailed information of TA system TA063999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 3917374..3917900
• Replicon: chromosome
• Accession: NZ_CP015964
• Organism: Mycobacterium intracellulare subsp. yongonense strain Asan 36912

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: S4ZDZ6
• Locus tag: MOTT12_RS18265
• Protein ID: WP_008259048.1
• Coordinates: 3917598..3917900 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: S4ZFD0
• Locus tag: MOTT12_RS18260
• Protein ID: WP_008259047.1
• Coordinates: 3917374..3917601 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOTT12_RS18235	3912451..3913371	-	921	WP_008259041.1	MinD/ParA family protein	-
MOTT12_RS18240	3913616..3914647	-	1032	WP_008259042.1	6-phosphofructokinase	-
MOTT12_RS18245	3914691..3916184	-	1494	WP_008259043.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
MOTT12_RS18250	3916181..3916480	-	300	WP_009952003.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
MOTT12_RS18255	3916580..3917236	+	657	WP_085977602.1	amino acid-binding protein	-
MOTT12_RS18260	3917374..3917601	+	228	WP_008259047.1	ribbon-helix-helix protein, CopG family	Antitoxin
MOTT12_RS18265	3917598..3917900	+	303	WP_008259048.1	hypothetical protein	Toxin
MOTT12_RS18270	3918016..3918324	-	309	WP_008259049.1	hypothetical protein	-
MOTT12_RS18275	3918383..3921304	-	2922	WP_036453523.1	MMPL family transporter	-
MOTT12_RS18280	3921301..3921747	-	447	WP_193375010.1	transport acessory protein MmpS	-
MOTT12_RS18285	3921952..3922470	+	519	WP_020823253.1	pyridoxamine 5'-phosphate oxidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10473.31 Da
• Isoelectric Point: 4.5636

>T63999 WP_008259048.1 NZ_CP015964:3917598-3917900 [Mycobacterium intracellulare subsp. yongonense]
MIAPGDIAPRRDTRRDLYVAVLSNAIHLAANTGRVIVCPFIPGKIPDDTMAMVVPLTQPEGTLLPELVQWLPTSALGEPI
GAVSAAALSEAMSIVTALIT

Nucleotide

• Length: 303 bp

>T63999 NZ_CP015964:3917598-3917900 [Mycobacterium intracellulare subsp. yongonense]
ATGATCGCGCCGGGCGACATCGCGCCGCGGCGAGACACCCGCCGCGACCTCTACGTCGCGGTGCTCTCCAACGCGATCCA
CCTCGCGGCCAACACTGGTCGCGTCATCGTCTGCCCGTTCATCCCTGGGAAAATCCCCGACGACACGATGGCCATGGTCG
TTCCGCTCACGCAGCCCGAAGGCACTCTTCTCCCCGAACTGGTGCAGTGGCTGCCGACTTCGGCGCTCGGTGAACCGATC
GGGGCCGTGTCGGCGGCCGCGCTTAGCGAGGCGATGTCGATCGTGACCGCGCTGATCACGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.14 Da
• Isoelectric Point: 6.4608

>AT63999 WP_008259047.1 NZ_CP015964:3917374-3917601 [Mycobacterium intracellulare subsp. yongonense]
MAKEKISVTVDAAVLADADSDAKAAGLNRSEMIERALRNEHLRRALHTYTTRTAPALNIDSYAGQVYEANRAADL

Nucleotide

• Length: 228 bp

>AT63999 NZ_CP015964:3917374-3917601 [Mycobacterium intracellulare subsp. yongonense]
ATGGCCAAAGAGAAGATTTCTGTCACCGTCGACGCCGCCGTGCTCGCCGACGCCGACTCCGACGCCAAAGCGGCCGGACT
GAATCGCTCCGAGATGATCGAGCGGGCGCTGCGCAATGAGCATCTACGTCGCGCGCTTCATACCTATACGACGCGGACCG
CACCAGCGCTCAACATCGACTCCTACGCCGGGCAAGTGTATGAAGCCAACCGGGCCGCGGACCTATGA

Structures

Toxin

• AlphaFold DB: S4ZDZ6

Antitoxin

• AlphaFold DB: S4ZFD0