Detailed information of TA system TA063997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1147407..1147969
• Replicon: chromosome
• Accession: NZ_CP015964
• Organism: Mycobacterium intracellulare subsp. yongonense strain Asan 36912

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A081I0A5
• Locus tag: MOTT12_RS05765
• Protein ID: WP_036454801.1
• Coordinates: 1147601..1147969 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A081I0A4
• Locus tag: MOTT12_RS05760
• Protein ID: WP_036455532.1
• Coordinates: 1147407..1147604 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOTT12_RS05725	1142825..1143565	+	741	WP_036454796.1	aminoglycoside 3'-phosphotransferase	-
MOTT12_RS05735	1144306..1145496	+	1191	WP_197095509.1	transposase	-
MOTT12_RS05740	1145704..1145988	+	285	WP_020821851.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MOTT12_RS05745	1146003..1146248	+	246	WP_036455529.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MOTT12_RS05750	1146284..1146835	+	552	WP_036454798.1	VOC family protein	-
MOTT12_RS05755	1146914..1147312	+	399	WP_036454800.1	VOC family protein	-
MOTT12_RS05760	1147407..1147604	+	198	WP_036455532.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MOTT12_RS05765	1147601..1147969	+	369	WP_036454801.1	type II toxin-antitoxin system VapC family toxin	Toxin
MOTT12_RS05770	1148152..1148493	+	342	WP_179945069.1	hypothetical protein	-
MOTT12_RS25710	1148579..1148938	+	360	WP_042910935.1	hypothetical protein	-
MOTT12_RS05780	1149123..1149698	+	576	WP_075630262.1	C40 family peptidase	-
MOTT12_RS05785	1150060..1150383	+	324	WP_036454806.1	antibiotic biosynthesis monooxygenase	-
MOTT12_RS05790	1150421..1151254	-	834	WP_036454808.1	SAM-dependent methyltransferase	-
MOTT12_RS05795	1151289..1151972	-	684	WP_036455537.1	hypothetical protein	-
MOTT12_RS05800	1152119..1152328	+	210	WP_072501223.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13435.74 Da
• Isoelectric Point: 6.4862

>T63997 WP_036454801.1 NZ_CP015964:1147601-1147969 [Mycobacterium intracellulare subsp. yongonense]
MILVDTAVWIDHLHTAELRLVKLLEIDQVGCHALVIEELALGSISRREVVLDLLANLRQFPTVQHAEILHLTGQRKLYGR
GLSAVDVHLMAAVALVEGAQLWTRDKRLKAASVDAGIALFDA

Nucleotide

• Length: 369 bp

>T63997 NZ_CP015964:1147601-1147969 [Mycobacterium intracellulare subsp. yongonense]
ATGATCCTCGTTGACACCGCGGTGTGGATCGATCATCTACACACCGCGGAGTTGCGGCTAGTCAAGCTGCTGGAGATCGA
CCAAGTTGGCTGTCATGCGCTGGTCATCGAAGAGCTGGCGCTGGGGTCGATCTCGCGACGTGAGGTGGTGCTCGACCTCC
TCGCGAATCTTCGCCAGTTTCCGACCGTCCAGCACGCCGAGATCCTTCACCTCACCGGTCAACGCAAACTGTATGGACGC
GGTCTCAGTGCGGTCGACGTCCATCTCATGGCCGCCGTCGCCCTGGTTGAAGGCGCGCAGCTGTGGACGAGAGACAAGCG
GTTGAAGGCTGCGTCGGTCGATGCGGGCATCGCACTCTTCGATGCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6871.88 Da
• Isoelectric Point: 11.6072

>AT63997 WP_036455532.1 NZ_CP015964:1147407-1147604 [Mycobacterium intracellulare subsp. yongonense]
MRTTVTIDDDLLAKAAELTGVRENVALLREGLQTLIRVESARRLAALGGTDAGAVAAPRRRRGTG

Nucleotide

• Length: 198 bp

>AT63997 NZ_CP015964:1147407-1147604 [Mycobacterium intracellulare subsp. yongonense]
ATGCGGACCACAGTCACGATCGACGACGACCTGTTGGCCAAGGCTGCTGAGTTGACCGGCGTGCGCGAGAACGTCGCGTT
GCTCCGCGAGGGATTGCAGACGCTGATCAGGGTGGAAAGCGCTCGCCGACTTGCCGCGCTCGGCGGGACGGATGCCGGGG
CGGTGGCCGCTCCCCGGCGCCGCCGTGGGACTGGATGA

Structures

Toxin

• AlphaFold DB: A0A081I0A5

Antitoxin

• AlphaFold DB: A0A081I0A4