Detailed information of TA system TA063993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1010706..1011328
• Replicon: chromosome
• Accession: NZ_CP015961
• Organism: Dietzia timorensis strain ID05-A0528

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A173LKB5
• Locus tag: BJL86_RS04675
• Protein ID: WP_067475746.1
• Coordinates: 1010933..1011328 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BJL86_RS04670
• Protein ID: WP_067475774.1
• Coordinates: 1010706..1010936 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJL86_RS04655	1006715..1007206	-	492	WP_067475740.1	SRPBCC family protein	-
BJL86_RS17060	1007581..1008309	-	729	WP_156515356.1	hypothetical protein	-
BJL86_RS04665	1008318..1010699	-	2382	WP_082908582.1	AAA family ATPase	-
BJL86_RS04670	1010706..1010936	+	231	WP_067475774.1	hypothetical protein	Antitoxin
BJL86_RS04675	1010933..1011328	+	396	WP_067475746.1	PIN domain-containing protein	Toxin
BJL86_RS04680	1011517..1012602	+	1086	WP_067475749.1	S-(hydroxymethyl)mycothiol dehydrogenase	-
BJL86_RS04685	1012635..1013294	+	660	WP_067475751.1	MBL fold metallo-hydrolase	-
BJL86_RS04690	1013388..1014329	-	942	WP_082908583.1	alpha/beta hydrolase	-
BJL86_RS04695	1014374..1015252	-	879	WP_067475754.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14515.54 Da
• Isoelectric Point: 4.9499

>T63993 WP_067475746.1 NZ_CP015961:1010933-1011328 [Dietzia timorensis]
MSERYLLDANALIALAIVEHEHHERAARWSLGAAEILLCPVVEGALCRFLVRSGVRGNDARELLQAFYGEPRISFCRDSI
SYCDVDLDHVVGHRQMADAYLASLAEETGSRLATFDKTLVGERPDVATMIP

Nucleotide

• Length: 396 bp

>T63993 NZ_CP015961:1010933-1011328 [Dietzia timorensis]
ATGAGCGAAAGGTATCTGCTCGATGCGAATGCGCTCATTGCCTTAGCAATCGTCGAGCACGAACACCACGAGCGCGCGGC
TCGCTGGTCGCTGGGAGCTGCAGAAATTCTGCTGTGTCCCGTGGTCGAGGGTGCGCTGTGTCGCTTTCTAGTTCGGAGTG
GAGTTCGAGGCAACGACGCTCGCGAATTGCTCCAAGCGTTCTATGGCGAGCCGCGCATTTCGTTCTGCCGAGATTCGATC
TCCTATTGCGATGTCGACCTGGACCACGTGGTCGGACATCGCCAGATGGCAGATGCCTACCTGGCGTCATTGGCAGAGGA
GACCGGTTCACGGCTCGCGACCTTCGATAAAACTTTGGTCGGTGAGCGCCCCGATGTGGCGACGATGATTCCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8200.25 Da
• Isoelectric Point: 4.7672

>AT63993 WP_067475774.1 NZ_CP015961:1010706-1010936 [Dietzia timorensis]
MRTTIDLPDAVHRRAKELAQARGESLSSVVADLAVKGLAAAGEPMRINRDPVSGLPTVNLGYRLTSEEVARELDDE

Nucleotide

• Length: 231 bp

>AT63993 NZ_CP015961:1010706-1010936 [Dietzia timorensis]
ATGCGTACGACAATTGATCTGCCCGACGCGGTGCACCGACGGGCCAAGGAGCTAGCACAGGCCCGAGGGGAATCGCTGTC
CTCCGTGGTCGCAGATCTTGCGGTCAAAGGGTTGGCAGCCGCCGGCGAACCCATGCGGATCAACCGCGACCCAGTCAGTG
GGCTTCCGACTGTGAATCTCGGCTACCGTCTAACTTCCGAAGAGGTCGCTCGAGAGCTCGACGACGAATGA

Structures

Toxin

• AlphaFold DB: A0A173LKB5