Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 15860..16458 Replicon plasmid unnamed1
Accession NZ_CP015957
Organism Legionella pneumophila strain E9_O

Toxin (Protein)


Gene name hicA Uniprot ID A0A2Z3L5E4
Locus tag A9E96_RS15020 Protein ID WP_019232989.1
Coordinates 16261..16458 (-) Length 66 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2Z3KTJ8
Locus tag A9E96_RS15015 Protein ID WP_019232990.1
Coordinates 15860..16264 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E96_RS14995 12569..12865 + 297 WP_019232994.1 hypothetical protein -
A9E96_RS15725 12881..13138 + 258 WP_127908205.1 hypothetical protein -
A9E96_RS15615 13150..13386 + 237 WP_080480132.1 hypothetical protein -
A9E96_RS15000 13699..14004 - 306 WP_019232993.1 helix-turn-helix domain-containing protein -
A9E96_RS15005 13997..14359 - 363 WP_019232992.1 type II toxin-antitoxin system RelE/ParE family toxin -
A9E96_RS15010 14523..15845 + 1323 WP_019232991.1 DUF1186 domain-containing protein -
A9E96_RS15015 15860..16264 - 405 WP_019232990.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A9E96_RS15020 16261..16458 - 198 WP_019232989.1 type II toxin-antitoxin system HicA family toxin Toxin
A9E96_RS15025 16628..16954 - 327 WP_019232988.1 hypothetical protein -
A9E96_RS15030 16978..17832 - 855 WP_080480134.1 site-specific integrase -
A9E96_RS15730 17836..17997 - 162 Protein_21 site-specific integrase -
A9E96_RS15035 18088..18315 - 228 WP_019232986.1 ChaB family protein -
A9E96_RS15040 18630..19379 - 750 WP_019232985.1 mechanosensitive ion channel family protein -
A9E96_RS15045 19836..21059 + 1224 WP_019232984.1 PAS domain S-box protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48354 48354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 66 a.a.        Molecular weight: 7401.77 Da        Isoelectric Point: 10.6519

>T63989 WP_019232989.1 NZ_CP015957:c16458-16261 [Legionella pneumophila]
MKSAEIIKIIERDGWVQCHQKGSHCQFKHPAKKGRVTVPHPKKDLPIKTVVSIFKQAGINKEDIK

Download         Length: 198 bp

>T63989 NZ_CP015957:c16458-16261 [Legionella pneumophila]
ATGAAAAGCGCAGAAATAATAAAAATAATAGAACGCGATGGTTGGGTGCAATGTCACCAGAAGGGGAGTCATTGTCAATT
TAAACATCCCGCCAAAAAGGGCAGGGTGACAGTGCCTCATCCTAAAAAAGATTTACCTATTAAAACGGTGGTCAGTATCT
TTAAGCAGGCTGGTATAAATAAGGAGGATATAAAATGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14964.88 Da        Isoelectric Point: 4.4085

>AT63989 WP_019232990.1 NZ_CP015957:c16264-15860 [Legionella pneumophila]
MRYPVVIHKDEQSDFGVSIPDIPGCYSAGSTYDEALTNAIEAIECHLEGLLMDNESLPVGTTIDRWINDEEFQGGVWAFI
DIDLSQISGKAKRINITMPERVLSLIDLYAKNHSIKNRSSFLADAALSYMESHK

Download         Length: 405 bp

>AT63989 NZ_CP015957:c16264-15860 [Legionella pneumophila]
ATGAGATATCCTGTAGTGATCCATAAAGATGAACAGAGTGATTTTGGTGTGAGTATCCCCGATATTCCTGGGTGCTATTC
TGCTGGTAGCACTTATGATGAAGCATTAACCAATGCTATTGAGGCGATTGAATGCCATCTCGAAGGGTTGTTAATGGATA
ATGAGTCCCTTCCGGTAGGTACAACAATTGATCGATGGATTAATGATGAAGAGTTTCAGGGTGGCGTATGGGCATTTATT
GACATTGATTTAAGCCAAATATCAGGTAAGGCAAAACGCATTAATATCACTATGCCAGAGCGGGTTTTAAGCCTGATTGA
TTTGTATGCTAAAAATCATTCTATAAAAAATCGCTCTTCTTTTTTAGCAGATGCAGCACTTAGCTATATGGAAAGTCATA
AATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3L5E4

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3KTJ8

References