Detailed information of TA system TA063982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2688353..2689809
• Replicon: chromosome
• Accession: NZ_CP015954
• Organism: Legionella pneumophila strain E7_O

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A0W0UKG6
• Locus tag: A9E94_RS11875
• Protein ID: WP_044496813.1
• Coordinates: 2688353..2689204 (-)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: A9E94_RS11880
• Protein ID: WP_044496821.1
• Coordinates: 2689201..2689809 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E94_RS15560	2683385..2685019	+	1635	WP_080444600.1	transposase	-
A9E94_RS11855	2684974..2685366	-	393	WP_040146985.1	IS66 family insertion sequence element accessory protein TnpB	-
A9E94_RS15990	2685428..2685632	-	205	Protein_2332	hypothetical protein	-
A9E94_RS11865	2685848..2686090	-	243	WP_058450390.1	hypothetical protein	-
A9E94_RS11870	2686333..2688273	+	1941	WP_061466923.1	DUF2779 domain-containing protein	-
A9E94_RS11875	2688353..2689204	-	852	WP_044496813.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A9E94_RS11880	2689201..2689809	-	609	WP_044496821.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
A9E94_RS15790	2690348..2690620	+	273	Protein_2337	transposase	-
A9E94_RS11885	2690617..2691021	+	405	WP_058450388.1	IS3 family transposase	-
A9E94_RS15575	2690979..2691104	+	126	WP_080444601.1	IS3 family transposase	-
A9E94_RS11890	2691229..2692281	+	1053	WP_044496812.1	LD-carboxypeptidase	-
A9E94_RS11895	2692288..2693505	-	1218	WP_061466925.1	MFS transporter	-
A9E94_RS11900	2693535..2694494	-	960	WP_145923833.1	ATP-dependent Clp protease ATP-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2640345..2696423	56078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31759.66 Da
• Isoelectric Point: 6.2796

>T63982 WP_044496813.1 NZ_CP015954:c2689204-2688353 [Legionella pneumophila]
MTKNIGESVRSRLKNIAIKEGSDFNAILTRYGLERLLYRIGESEYSNQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICSISADDGINFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLN
DLPAPKIRTYPIYTVIAEKLHAIALLGMANSRLKDYLDLYVLLSNEQLDNQVLAKAIQATFTRRGMAIPEELPIGLTDEF
ANDPSRESMWKAFLRKNELEQKPLTEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

>T63982 NZ_CP015954:c2689204-2688353 [Legionella pneumophila]
ATGACAAAAAATATTGGTGAGTCTGTGCGCTCACGTTTGAAGAATATAGCTATAAAAGAAGGGTCTGATTTTAACGCGAT
ATTGACTCGTTACGGACTAGAACGTTTGTTATATCGGATTGGAGAATCAGAATATTCTAATCAGTTCTTGTTGAAGGGTG
CTTTACTTTTTAACCTATGGTATGACATGCCACATCGACCCACTAAAGATATTGATTTATTGGGGTTTGGTGATAATGAT
CTAGCGTATATTAAACAAACGTTTAGTAAAATCTGCAGCATCTCCGCAGATGATGGAATAAACTTTTTATCTGAATCAGT
GACGGTAGACACTATAAAAAAAGATGGTGGGTATACTGGAGCAAGAGTTGAATTATTTGGGGAATTAGCTAAAGCAAAGA
TTAAAATACAAATTGATATTGGTTATGGTGATGCAGTTACACCTGGTCCAATAGATGCCCATTATCCCGTTCTCCTTAAT
GATCTTCCTGCTCCTAAGATCCGTACTTATCCAATTTATACGGTAATCGCAGAAAAATTGCATGCTATTGCATTATTGGG
CATGGCTAATAGTCGGTTAAAAGACTACTTGGATCTTTATGTGTTATTAAGTAATGAGCAGTTAGATAATCAAGTCTTGG
CTAAAGCTATACAGGCTACATTTACTCGTCGAGGTATGGCTATTCCTGAAGAGCTGCCTATTGGTCTGACTGATGAGTTT
GCTAATGATCCAAGCAGAGAGTCGATGTGGAAAGCCTTTTTGAGAAAAAATGAACTAGAGCAGAAACCATTAACTGAAGT
TATTGCTGTGATCAGAAATTTGATTCAAGTGCCATATTCTTTGGCAAAGTAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 23043.21 Da
• Isoelectric Point: 9.7388

>AT63982 WP_044496821.1 NZ_CP015954:c2689809-2689201 [Legionella pneumophila]
MKTKDSYKQIVLQMARKTGVIKMSDLTKQGITRATVSRLVNENKLEKLAPGLYCLPETKFSEKESLIIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHVPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYKCAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT63982 NZ_CP015954:c2689809-2689201 [Legionella pneumophila]
ATGAAGACAAAGGATAGCTATAAACAAATAGTACTCCAAATGGCTAGAAAAACGGGTGTAATCAAAATGTCCGATCTAAC
CAAACAGGGGATAACTAGAGCGACCGTTTCAAGACTTGTTAACGAAAACAAACTTGAAAAACTTGCTCCCGGACTATATT
GCCTACCCGAGACTAAGTTTTCTGAAAAGGAAAGCCTGATTATTATAGCAAGCCGAGTACCTCAAGCCGTGTTTTGTCTG
CTAACTGCTTTGCAGATCCATGATCTAACAACACAACTACCTCGAAAAGTATGGATCGCTATGCCAAAGGGAAGTCATGT
GCCAAAAATGGATTATCCGCCGCTAAAAATGGTGCAATATTCAGATGAAGCCTTTAGTGAAGGAATTGAAATTATAGAAT
CAGATAATATTAAACTAAGGGTGTATAAATGTGCCAAGACAATTGCAGATTGTTTCAAACATCGCAATAAAATTGGACTT
GATGTGGCCATTGAAGCGCTCAAAGAGGCTTATACAAAAAATAAAGTAACAGTTGATGAACTATGGCATTATGCGAAAAT
TTGTCGCGTTGCAAACGTAATGCGTCCTTATATTGAGGCAATTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0W0UKG6