Detailed information of TA system TA063979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 46135..46733
• Replicon: plasmid unnamed1
• Accession: NZ_CP015952
• Organism: Legionella pneumophila strain E5_N

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: A9E92_RS15440
• Protein ID: WP_058475085.1
• Coordinates: 46536..46733 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378P8P3
• Locus tag: A9E92_RS15435
• Protein ID: WP_058475084.1
• Coordinates: 46135..46539 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E92_RS15410	41549..41872	+	324	WP_061363910.1	hypothetical protein	-
A9E92_RS16130	42213..42323	-	111	WP_158513232.1	(2Fe-2S)-binding protein	-
A9E92_RS16135	42381..42743	+	363	WP_129462639.1	hypothetical protein	-
A9E92_RS15420	42782..43135	+	354	WP_061363912.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A9E92_RS15425	43113..43403	+	291	WP_021460513.1	helix-turn-helix domain-containing protein	-
A9E92_RS15430	44416..46110	-	1695	WP_061363913.1	hypothetical protein	-
A9E92_RS15435	46135..46539	-	405	WP_058475084.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A9E92_RS15440	46536..46733	-	198	WP_058475085.1	type II toxin-antitoxin system HicA family toxin	Toxin
A9E92_RS15445	46916..47800	+	885	WP_061363914.1	recombinase family protein	-
A9E92_RS15450	47800..48261	+	462	WP_061363915.1	WGR domain-containing protein	-
A9E92_RS15455	48279..49643	-	1365	WP_061363916.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73576	73576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7389.76 Da
• Isoelectric Point: 10.7302

>T63979 WP_058475085.1 NZ_CP015952:c46733-46536 [Legionella pneumophila]
MKSAEIIKIIERDGWVKCHQKGSHCQFKHPTKKGRVTVPHPKKDLPVKTVASIFKQAGINKEDIK

Nucleotide

• Length: 198 bp

>T63979 NZ_CP015952:c46733-46536 [Legionella pneumophila]
ATGAAAAGCGCAGAAATAATAAAAATAATAGAACGCGATGGTTGGGTGAAGTGTCACCAGAAAGGGAGTCATTGCCAATT
TAAGCATCCAACAAAAAAGGGTAGGGTGACTGTGCCTCATCCTAAAAAAGATTTACCCGTTAAAACGGTAGCTAGTATTT
TTAAACAGGCTGGAATTAATAAGGAGGATATAAAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14961.80 Da
• Isoelectric Point: 4.5209

>AT63979 WP_058475084.1 NZ_CP015952:c46539-46135 [Legionella pneumophila]
MRYPVVIHKDEHSDYGVSVPDIPGCYSAGATYDEALTNVIEAIECHLEGLLMDNESIPVGSTIDHWINEEEFQGGVWAFI
DIDLSQISGKSKRINITIPERVLNLIDLYSKNHAVKNRSSFLADAALSYIESHK

Nucleotide

• Length: 405 bp

>AT63979 NZ_CP015952:c46539-46135 [Legionella pneumophila]
ATGAGATATCCTGTAGTAATACATAAAGACGAGCACAGTGATTATGGCGTAAGTGTCCCAGATATTCCTGGATGTTATTC
TGCTGGAGCTACTTATGATGAGGCGTTAACTAACGTCATTGAGGCAATTGAATGTCATTTAGAAGGGTTGTTAATGGACA
ATGAGTCCATACCAGTAGGTTCTACAATTGATCATTGGATAAATGAGGAAGAGTTTCAGGGTGGAGTGTGGGCATTTATT
GATATTGATTTAAGTCAAATATCAGGTAAATCAAAACGCATCAATATCACTATTCCAGAGCGCGTATTAAATCTTATTGA
TTTATATTCTAAAAATCATGCAGTAAAAAATCGCTCTTCATTTTTAGCAGATGCAGCACTTAGTTATATAGAAAGTCATA
AATAA

Structures

Antitoxin

• AlphaFold DB: A0A378P8P3