Detailed information of TA system TA063975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2680745..2682201
• Replicon: chromosome
• Accession: NZ_CP015950
• Organism: Legionella pneumophila strain E4_N

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A0W0UKG6
• Locus tag: A9E91_RS11820
• Protein ID: WP_044496813.1
• Coordinates: 2680745..2681596 (-)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: A9E91_RS11825
• Protein ID: WP_044496821.1
• Coordinates: 2681593..2682201 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E91_RS15490	2675777..2677411	+	1635	WP_080444600.1	transposase	-
A9E91_RS11800	2677366..2677758	-	393	WP_040146985.1	IS66 family insertion sequence element accessory protein TnpB	-
A9E91_RS15835	2677820..2678024	-	205	Protein_2318	hypothetical protein	-
A9E91_RS11810	2678240..2678482	-	243	WP_058450390.1	hypothetical protein	-
A9E91_RS11815	2678725..2680665	+	1941	WP_061466923.1	DUF2779 domain-containing protein	-
A9E91_RS11820	2680745..2681596	-	852	WP_044496813.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A9E91_RS11825	2681593..2682201	-	609	WP_044496821.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
A9E91_RS15685	2682740..2683012	+	273	Protein_2323	transposase	-
A9E91_RS11830	2683009..2683413	+	405	WP_058450388.1	IS3 family transposase	-
A9E91_RS15505	2683371..2683496	+	126	WP_080444601.1	IS3 family transposase	-
A9E91_RS11835	2683621..2684673	+	1053	WP_044496812.1	LD-carboxypeptidase	-
A9E91_RS11840	2684680..2685897	-	1218	WP_061466925.1	MFS transporter	-
A9E91_RS11845	2685927..2686973	-	1047	WP_052585449.1	ATP-dependent Clp protease ATP-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2632737..2688815	56078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31759.66 Da
• Isoelectric Point: 6.2796

>T63975 WP_044496813.1 NZ_CP015950:c2681596-2680745 [Legionella pneumophila]
MTKNIGESVRSRLKNIAIKEGSDFNAILTRYGLERLLYRIGESEYSNQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICSISADDGINFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLN
DLPAPKIRTYPIYTVIAEKLHAIALLGMANSRLKDYLDLYVLLSNEQLDNQVLAKAIQATFTRRGMAIPEELPIGLTDEF
ANDPSRESMWKAFLRKNELEQKPLTEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

>T63975 NZ_CP015950:c2681596-2680745 [Legionella pneumophila]
ATGACAAAAAATATTGGTGAGTCTGTGCGCTCACGTTTGAAGAATATAGCTATAAAAGAAGGGTCTGATTTTAACGCGAT
ATTGACTCGTTACGGACTAGAACGTTTGTTATATCGGATTGGAGAATCAGAATATTCTAATCAGTTCTTGTTGAAGGGTG
CTTTACTTTTTAACCTATGGTATGACATGCCACATCGACCCACTAAAGATATTGATTTATTGGGGTTTGGTGATAATGAT
CTAGCGTATATTAAACAAACGTTTAGTAAAATCTGCAGCATCTCCGCAGATGATGGAATAAACTTTTTATCTGAATCAGT
GACGGTAGACACTATAAAAAAAGATGGTGGGTATACTGGAGCAAGAGTTGAATTATTTGGGGAATTAGCTAAAGCAAAGA
TTAAAATACAAATTGATATTGGTTATGGTGATGCAGTTACACCTGGTCCAATAGATGCCCATTATCCCGTTCTCCTTAAT
GATCTTCCTGCTCCTAAGATCCGTACTTATCCAATTTATACGGTAATCGCAGAAAAATTGCATGCTATTGCATTATTGGG
CATGGCTAATAGTCGGTTAAAAGACTACTTGGATCTTTATGTGTTATTAAGTAATGAGCAGTTAGATAATCAAGTCTTGG
CTAAAGCTATACAGGCTACATTTACTCGTCGAGGTATGGCTATTCCTGAAGAGCTGCCTATTGGTCTGACTGATGAGTTT
GCTAATGATCCAAGCAGAGAGTCGATGTGGAAAGCCTTTTTGAGAAAAAATGAACTAGAGCAGAAACCATTAACTGAAGT
TATTGCTGTGATCAGAAATTTGATTCAAGTGCCATATTCTTTGGCAAAGTAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 23043.21 Da
• Isoelectric Point: 9.7388

>AT63975 WP_044496821.1 NZ_CP015950:c2682201-2681593 [Legionella pneumophila]
MKTKDSYKQIVLQMARKTGVIKMSDLTKQGITRATVSRLVNENKLEKLAPGLYCLPETKFSEKESLIIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHVPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYKCAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT63975 NZ_CP015950:c2682201-2681593 [Legionella pneumophila]
ATGAAGACAAAGGATAGCTATAAACAAATAGTACTCCAAATGGCTAGAAAAACGGGTGTAATCAAAATGTCCGATCTAAC
CAAACAGGGGATAACTAGAGCGACCGTTTCAAGACTTGTTAACGAAAACAAACTTGAAAAACTTGCTCCCGGACTATATT
GCCTACCCGAGACTAAGTTTTCTGAAAAGGAAAGCCTGATTATTATAGCAAGCCGAGTACCTCAAGCCGTGTTTTGTCTG
CTAACTGCTTTGCAGATCCATGATCTAACAACACAACTACCTCGAAAAGTATGGATCGCTATGCCAAAGGGAAGTCATGT
GCCAAAAATGGATTATCCGCCGCTAAAAATGGTGCAATATTCAGATGAAGCCTTTAGTGAAGGAATTGAAATTATAGAAT
CAGATAATATTAAACTAAGGGTGTATAAATGTGCCAAGACAATTGCAGATTGTTTCAAACATCGCAATAAAATTGGACTT
GATGTGGCCATTGAAGCGCTCAAAGAGGCTTATACAAAAAATAAAGTAACAGTTGATGAACTATGGCATTATGCGAAAAT
TTGTCGCGTTGCAAACGTAATGCGTCCTTATATTGAGGCAATTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0W0UKG6