Detailed information of TA system TA063973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2727713..2729169
• Replicon: chromosome
• Accession: NZ_CP015949
• Organism: Legionella pneumophila strain E3_N

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A0W0UKG6
• Locus tag: A9E90_RS12085
• Protein ID: WP_044496813.1
• Coordinates: 2727713..2728564 (-)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: A9E90_RS12090
• Protein ID: WP_044496821.1
• Coordinates: 2728561..2729169 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E90_RS15775	2722745..2724379	+	1635	WP_080444600.1	transposase	-
A9E90_RS12065	2724334..2724726	-	393	WP_040146985.1	IS66 family insertion sequence element accessory protein TnpB	-
A9E90_RS16150	2724788..2724992	-	205	Protein_2372	hypothetical protein	-
A9E90_RS12075	2725208..2725450	-	243	WP_058450390.1	hypothetical protein	-
A9E90_RS12080	2725693..2727633	+	1941	WP_061466923.1	DUF2779 domain-containing protein	-
A9E90_RS12085	2727713..2728564	-	852	WP_044496813.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A9E90_RS12090	2728561..2729169	-	609	WP_044496821.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
A9E90_RS15985	2729708..2729980	+	273	Protein_2377	transposase	-
A9E90_RS12095	2729977..2730381	+	405	WP_058450388.1	IS3 family transposase	-
A9E90_RS15790	2730339..2730464	+	126	WP_080444601.1	IS3 family transposase	-
A9E90_RS12100	2730589..2731641	+	1053	WP_044496812.1	LD-carboxypeptidase	-
A9E90_RS12105	2731648..2732865	-	1218	WP_061466925.1	MFS transporter	-
A9E90_RS12110	2732895..2733941	-	1047	WP_052585449.1	ATP-dependent Clp protease ATP-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2679705..2735783	56078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31759.66 Da
• Isoelectric Point: 6.2796

>T63973 WP_044496813.1 NZ_CP015949:c2728564-2727713 [Legionella pneumophila]
MTKNIGESVRSRLKNIAIKEGSDFNAILTRYGLERLLYRIGESEYSNQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICSISADDGINFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLN
DLPAPKIRTYPIYTVIAEKLHAIALLGMANSRLKDYLDLYVLLSNEQLDNQVLAKAIQATFTRRGMAIPEELPIGLTDEF
ANDPSRESMWKAFLRKNELEQKPLTEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

>T63973 NZ_CP015949:c2728564-2727713 [Legionella pneumophila]
ATGACAAAAAATATTGGTGAGTCTGTGCGCTCACGTTTGAAGAATATAGCTATAAAAGAAGGGTCTGATTTTAACGCGAT
ATTGACTCGTTACGGACTAGAACGTTTGTTATATCGGATTGGAGAATCAGAATATTCTAATCAGTTCTTGTTGAAGGGTG
CTTTACTTTTTAACCTATGGTATGACATGCCACATCGACCCACTAAAGATATTGATTTATTGGGGTTTGGTGATAATGAT
CTAGCGTATATTAAACAAACGTTTAGTAAAATCTGCAGCATCTCCGCAGATGATGGAATAAACTTTTTATCTGAATCAGT
GACGGTAGACACTATAAAAAAAGATGGTGGGTATACTGGAGCAAGAGTTGAATTATTTGGGGAATTAGCTAAAGCAAAGA
TTAAAATACAAATTGATATTGGTTATGGTGATGCAGTTACACCTGGTCCAATAGATGCCCATTATCCCGTTCTCCTTAAT
GATCTTCCTGCTCCTAAGATCCGTACTTATCCAATTTATACGGTAATCGCAGAAAAATTGCATGCTATTGCATTATTGGG
CATGGCTAATAGTCGGTTAAAAGACTACTTGGATCTTTATGTGTTATTAAGTAATGAGCAGTTAGATAATCAAGTCTTGG
CTAAAGCTATACAGGCTACATTTACTCGTCGAGGTATGGCTATTCCTGAAGAGCTGCCTATTGGTCTGACTGATGAGTTT
GCTAATGATCCAAGCAGAGAGTCGATGTGGAAAGCCTTTTTGAGAAAAAATGAACTAGAGCAGAAACCATTAACTGAAGT
TATTGCTGTGATCAGAAATTTGATTCAAGTGCCATATTCTTTGGCAAAGTAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 23043.21 Da
• Isoelectric Point: 9.7388

>AT63973 WP_044496821.1 NZ_CP015949:c2729169-2728561 [Legionella pneumophila]
MKTKDSYKQIVLQMARKTGVIKMSDLTKQGITRATVSRLVNENKLEKLAPGLYCLPETKFSEKESLIIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHVPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYKCAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT63973 NZ_CP015949:c2729169-2728561 [Legionella pneumophila]
ATGAAGACAAAGGATAGCTATAAACAAATAGTACTCCAAATGGCTAGAAAAACGGGTGTAATCAAAATGTCCGATCTAAC
CAAACAGGGGATAACTAGAGCGACCGTTTCAAGACTTGTTAACGAAAACAAACTTGAAAAACTTGCTCCCGGACTATATT
GCCTACCCGAGACTAAGTTTTCTGAAAAGGAAAGCCTGATTATTATAGCAAGCCGAGTACCTCAAGCCGTGTTTTGTCTG
CTAACTGCTTTGCAGATCCATGATCTAACAACACAACTACCTCGAAAAGTATGGATCGCTATGCCAAAGGGAAGTCATGT
GCCAAAAATGGATTATCCGCCGCTAAAAATGGTGCAATATTCAGATGAAGCCTTTAGTGAAGGAATTGAAATTATAGAAT
CAGATAATATTAAACTAAGGGTGTATAAATGTGCCAAGACAATTGCAGATTGTTTCAAACATCGCAATAAAATTGGACTT
GATGTGGCCATTGAAGCGCTCAAAGAGGCTTATACAAAAAATAAAGTAACAGTTGATGAACTATGGCATTATGCGAAAAT
TTGTCGCGTTGCAAACGTAATGCGTCCTTATATTGAGGCAATTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0W0UKG6