Detailed information of TA system TA063965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3542..4140
• Replicon: plasmid unamed2
• Accession: NZ_CP015943
• Organism: Legionella pneumophila strain C9_S

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: A9E85_RS15290
• Protein ID: WP_058475085.1
• Coordinates: 3943..4140 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378P8P3
• Locus tag: A9E85_RS15285
• Protein ID: WP_058475084.1
• Coordinates: 3542..3946 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E85_RS15270	189..542	+	354	WP_061363912.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A9E85_RS15275	520..810	+	291	WP_021460513.1	helix-turn-helix domain-containing protein	-
A9E85_RS15280	1823..3517	-	1695	WP_061363913.1	hypothetical protein	-
A9E85_RS15285	3542..3946	-	405	WP_058475084.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
A9E85_RS15290	3943..4140	-	198	WP_058475085.1	type II toxin-antitoxin system HicA family toxin	Toxin
A9E85_RS15295	4323..5207	+	885	WP_061363914.1	recombinase family protein	-
A9E85_RS15300	5207..5668	+	462	WP_061363915.1	WGR domain-containing protein	-
A9E85_RS15305	5686..7050	-	1365	WP_061363916.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73576	73576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7389.76 Da
• Isoelectric Point: 10.7302

>T63965 WP_058475085.1 NZ_CP015943:c4140-3943 [Legionella pneumophila]
MKSAEIIKIIERDGWVKCHQKGSHCQFKHPTKKGRVTVPHPKKDLPVKTVASIFKQAGINKEDIK

Nucleotide

• Length: 198 bp

>T63965 NZ_CP015943:c4140-3943 [Legionella pneumophila]
ATGAAAAGCGCAGAAATAATAAAAATAATAGAACGCGATGGTTGGGTGAAGTGTCACCAGAAAGGGAGTCATTGCCAATT
TAAGCATCCAACAAAAAAGGGTAGGGTGACTGTGCCTCATCCTAAAAAAGATTTACCCGTTAAAACGGTAGCTAGTATTT
TTAAACAGGCTGGAATTAATAAGGAGGATATAAAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14961.80 Da
• Isoelectric Point: 4.5209

>AT63965 WP_058475084.1 NZ_CP015943:c3946-3542 [Legionella pneumophila]
MRYPVVIHKDEHSDYGVSVPDIPGCYSAGATYDEALTNVIEAIECHLEGLLMDNESIPVGSTIDHWINEEEFQGGVWAFI
DIDLSQISGKSKRINITIPERVLNLIDLYSKNHAVKNRSSFLADAALSYIESHK

Nucleotide

• Length: 405 bp

>AT63965 NZ_CP015943:c3946-3542 [Legionella pneumophila]
ATGAGATATCCTGTAGTAATACATAAAGACGAGCACAGTGATTATGGCGTAAGTGTCCCAGATATTCCTGGATGTTATTC
TGCTGGAGCTACTTATGATGAGGCGTTAACTAACGTCATTGAGGCAATTGAATGTCATTTAGAAGGGTTGTTAATGGACA
ATGAGTCCATACCAGTAGGTTCTACAATTGATCATTGGATAAATGAGGAAGAGTTTCAGGGTGGAGTGTGGGCATTTATT
GATATTGATTTAAGTCAAATATCAGGTAAATCAAAACGCATCAATATCACTATTCCAGAGCGCGTATTAAATCTTATTGA
TTTATATTCTAAAAATCATGCAGTAAAAAATCGCTCTTCATTTTTAGCAGATGCAGCACTTAGTTATATAGAAAGTCATA
AATAA

Structures

Antitoxin

• AlphaFold DB: A0A378P8P3