Detailed information of TA system TA063954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2682089..2683545
• Replicon: chromosome
• Accession: NZ_CP015936
• Organism: Legionella pneumophila strain C5_P

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A0W0UKG6
• Locus tag: A9E81_RS11845
• Protein ID: WP_044496813.1
• Coordinates: 2682089..2682940 (-)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: A9E81_RS11850
• Protein ID: WP_044496821.1
• Coordinates: 2682937..2683545 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E81_RS15520	2677121..2678755	+	1635	WP_080444600.1	transposase	-
A9E81_RS11825	2678710..2679102	-	393	WP_040146985.1	IS66 family insertion sequence element accessory protein TnpB	-
A9E81_RS15865	2679164..2679368	-	205	Protein_2321	hypothetical protein	-
A9E81_RS11835	2679584..2679826	-	243	WP_058450390.1	hypothetical protein	-
A9E81_RS11840	2680069..2682009	+	1941	WP_061466923.1	DUF2779 domain-containing protein	-
A9E81_RS11845	2682089..2682940	-	852	WP_044496813.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A9E81_RS11850	2682937..2683545	-	609	WP_044496821.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
A9E81_RS15730	2684084..2684356	+	273	Protein_2326	transposase	-
A9E81_RS11855	2684353..2684757	+	405	WP_058450388.1	IS3 family transposase	-
A9E81_RS15535	2684715..2684840	+	126	WP_080444601.1	IS3 family transposase	-
A9E81_RS11860	2684965..2686017	+	1053	WP_044496812.1	LD-carboxypeptidase	-
A9E81_RS11865	2686024..2687241	-	1218	WP_061466925.1	MFS transporter	-
A9E81_RS11870	2687271..2688317	-	1047	WP_052585449.1	ATP-dependent Clp protease ATP-binding subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2634081..2690159	56078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31759.66 Da
• Isoelectric Point: 6.2796

>T63954 WP_044496813.1 NZ_CP015936:c2682940-2682089 [Legionella pneumophila]
MTKNIGESVRSRLKNIAIKEGSDFNAILTRYGLERLLYRIGESEYSNQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICSISADDGINFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLN
DLPAPKIRTYPIYTVIAEKLHAIALLGMANSRLKDYLDLYVLLSNEQLDNQVLAKAIQATFTRRGMAIPEELPIGLTDEF
ANDPSRESMWKAFLRKNELEQKPLTEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

>T63954 NZ_CP015936:c2682940-2682089 [Legionella pneumophila]
ATGACAAAAAATATTGGTGAGTCTGTGCGCTCACGTTTGAAGAATATAGCTATAAAAGAAGGGTCTGATTTTAACGCGAT
ATTGACTCGTTACGGACTAGAACGTTTGTTATATCGGATTGGAGAATCAGAATATTCTAATCAGTTCTTGTTGAAGGGTG
CTTTACTTTTTAACCTATGGTATGACATGCCACATCGACCCACTAAAGATATTGATTTATTGGGGTTTGGTGATAATGAT
CTAGCGTATATTAAACAAACGTTTAGTAAAATCTGCAGCATCTCCGCAGATGATGGAATAAACTTTTTATCTGAATCAGT
GACGGTAGACACTATAAAAAAAGATGGTGGGTATACTGGAGCAAGAGTTGAATTATTTGGGGAATTAGCTAAAGCAAAGA
TTAAAATACAAATTGATATTGGTTATGGTGATGCAGTTACACCTGGTCCAATAGATGCCCATTATCCCGTTCTCCTTAAT
GATCTTCCTGCTCCTAAGATCCGTACTTATCCAATTTATACGGTAATCGCAGAAAAATTGCATGCTATTGCATTATTGGG
CATGGCTAATAGTCGGTTAAAAGACTACTTGGATCTTTATGTGTTATTAAGTAATGAGCAGTTAGATAATCAAGTCTTGG
CTAAAGCTATACAGGCTACATTTACTCGTCGAGGTATGGCTATTCCTGAAGAGCTGCCTATTGGTCTGACTGATGAGTTT
GCTAATGATCCAAGCAGAGAGTCGATGTGGAAAGCCTTTTTGAGAAAAAATGAACTAGAGCAGAAACCATTAACTGAAGT
TATTGCTGTGATCAGAAATTTGATTCAAGTGCCATATTCTTTGGCAAAGTAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 23043.21 Da
• Isoelectric Point: 9.7388

>AT63954 WP_044496821.1 NZ_CP015936:c2683545-2682937 [Legionella pneumophila]
MKTKDSYKQIVLQMARKTGVIKMSDLTKQGITRATVSRLVNENKLEKLAPGLYCLPETKFSEKESLIIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHVPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYKCAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT63954 NZ_CP015936:c2683545-2682937 [Legionella pneumophila]
ATGAAGACAAAGGATAGCTATAAACAAATAGTACTCCAAATGGCTAGAAAAACGGGTGTAATCAAAATGTCCGATCTAAC
CAAACAGGGGATAACTAGAGCGACCGTTTCAAGACTTGTTAACGAAAACAAACTTGAAAAACTTGCTCCCGGACTATATT
GCCTACCCGAGACTAAGTTTTCTGAAAAGGAAAGCCTGATTATTATAGCAAGCCGAGTACCTCAAGCCGTGTTTTGTCTG
CTAACTGCTTTGCAGATCCATGATCTAACAACACAACTACCTCGAAAAGTATGGATCGCTATGCCAAAGGGAAGTCATGT
GCCAAAAATGGATTATCCGCCGCTAAAAATGGTGCAATATTCAGATGAAGCCTTTAGTGAAGGAATTGAAATTATAGAAT
CAGATAATATTAAACTAAGGGTGTATAAATGTGCCAAGACAATTGCAGATTGTTTCAAACATCGCAATAAAATTGGACTT
GATGTGGCCATTGAAGCGCTCAAAGAGGCTTATACAAAAAATAAAGTAACAGTTGATGAACTATGGCATTATGCGAAAAT
TTGTCGCGTTGCAAACGTAATGCGTCCTTATATTGAGGCAATTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0W0UKG6