Detailed information of TA system TA063946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2345700..2347421
• Replicon: chromosome
• Accession: NZ_CP015932
• Organism: Legionella pneumophila strain C1_S

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: A9E77_RS10440
• Protein ID: WP_038839099.1
• Coordinates: 2345700..2346632 (-)
• Length: 311 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A128I8L5
• Locus tag: A9E77_RS10445
• Protein ID: WP_015444253.1
• Coordinates: 2346636..2347421 (-)
• Length: 262 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E77_RS10415	2341259..2341612	-	354	WP_015444259.1	conjugal transfer transcriptional regulator TraJ	-
A9E77_RS10420	2342006..2342350	+	345	WP_015444258.1	TraK family protein	-
A9E77_RS10425	2342350..2343075	+	726	WP_015444257.1	IncP-type DNA transfer protein TraL	-
A9E77_RS10430	2343075..2343512	+	438	WP_015444256.1	conjugal transfer protein TraM	-
A9E77_RS10435	2343627..2345567	+	1941	WP_038839098.1	DUF2779 domain-containing protein	-
A9E77_RS10440	2345700..2346632	-	933	WP_038839099.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A9E77_RS10445	2346636..2347421	-	786	WP_015444253.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
A9E77_RS10450	2347906..2348910	-	1005	WP_010947770.1	site-specific integrase	-
A9E77_RS10455	2348823..2349125	-	303	WP_010947771.1	helix-turn-helix domain-containing protein	-
A9E77_RS10465	2349535..2350158	+	624	WP_010947772.1	helix-turn-helix domain-containing protein	-
A9E77_RS10470	2350155..2350361	-	207	WP_021436844.1	hypothetical protein	-
A9E77_RS10475	2350522..2351829	+	1308	WP_010947773.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2272402..2412050	139648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-264)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (97-254)

Sequences

Toxin

Protein

• Length: 311 a.a.
• Molecular weight: 35330.14 Da
• Isoelectric Point: 6.8643

>T63946 WP_038839099.1 NZ_CP015932:c2346632-2345700 [Legionella pneumophila]
MNKQSPYYKQVLLLIRLIPLVEKETCFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPREIALANVRNALAGIAANINKI
PDLNAVLQDNKPDEMRIIVEDHSSLNRGTQIKIEVSPVARGTLLPPVELDVVASVENEFGFASMKVVSLPDLYGGKLCAA
LDRQHPRDLFDVKVLLENQKIDRELFNGFLTYLLSHKRPISEIMNPRWKDITQVFEREFKGMTAEPVTLEELTAIPGKMI
QGLKDNFTQQDYDFLVSFKRGEPEWSLACSDQIQYLPAVQWKLLNIRKMPKQKLMDSVSLLQRTMGHWLN

Nucleotide

• Length: 933 bp

>T63946 NZ_CP015932:c2346632-2345700 [Legionella pneumophila]
ATGAATAAACAGTCACCATATTATAAACAGGTTTTGTTGCTCATTCGTTTAATTCCACTCGTCGAAAAGGAAACTTGTTT
TGCTTTAAAGGGAGGTACTGCCATTAATTTATTTGTGCGAGATTTCCCGAGGTTATCTGTGGATATAGATCTAGCGTATT
TACCCCTCGAGCCACGTGAGATTGCTTTAGCCAATGTGCGTAATGCATTAGCGGGTATTGCGGCAAATATTAATAAAATT
CCAGATTTGAATGCAGTTTTGCAAGATAACAAACCAGATGAAATGCGAATTATTGTTGAAGATCATTCTTCTCTAAATAG
GGGGACCCAAATCAAGATAGAAGTTTCACCTGTTGCGCGTGGAACTCTTTTGCCGCCAGTTGAGCTTGATGTTGTTGCTT
CGGTGGAAAATGAGTTTGGTTTTGCGTCAATGAAAGTCGTTTCTTTGCCTGATCTCTATGGTGGTAAATTATGCGCTGCT
TTAGATCGCCAGCATCCTAGAGATCTTTTTGATGTTAAGGTTTTGTTAGAAAATCAAAAGATTGACCGAGAGCTTTTTAA
TGGGTTTCTTACTTATCTTTTAAGTCACAAAAGGCCTATTTCAGAAATAATGAATCCACGATGGAAAGATATAACTCAAG
TTTTTGAACGTGAATTTAAAGGAATGACTGCTGAACCAGTTACCCTGGAAGAGCTAACAGCAATACCAGGAAAAATGATT
CAGGGTCTTAAAGATAATTTTACTCAGCAGGATTATGATTTTTTAGTCTCTTTTAAAAGGGGCGAGCCTGAGTGGTCTCT
TGCATGTAGCGATCAGATTCAATATCTCCCAGCAGTACAATGGAAGCTTTTAAATATTCGCAAAATGCCCAAACAAAAAC
TCATGGATTCAGTTAGCTTGCTACAAAGAACGATGGGACATTGGCTTAATTAG

Antitoxin

Protein

• Length: 262 a.a.
• Molecular weight: 29773.22 Da
• Isoelectric Point: 9.4457

>AT63946 WP_015444253.1 NZ_CP015932:c2347421-2346636 [Legionella pneumophila]
MSSKINWLLNNIASGEILLQSWLSSHGITPQLARKYCQSQWLVKLGVGVYYRAGRKPRWQNAVYCLQNQSRYMIHLAGLT
SLAQQGKSHYLQFAPDSIWINVQAKTILPKWFKNFPELVISDELIPNWEIIKTSKLNAQDENDLTTIEVDGIKLKASNQE
LAAFEVLEAVPSTLSFEHAAELFQGLVNLSPRKVEHILKRSGSIQTNRLFLFLGHFYKHPWAERVNESLINLGAGKRQIV
PDGKLEKQYQITVPEQFISSD

Nucleotide

• Length: 786 bp

>AT63946 NZ_CP015932:c2347421-2346636 [Legionella pneumophila]
ATGTCATCAAAAATAAACTGGCTTCTGAATAATATTGCCTCAGGTGAAATCTTGCTCCAGTCTTGGTTATCAAGCCATGG
AATTACTCCACAATTAGCAAGAAAATATTGTCAAAGCCAATGGTTGGTAAAATTGGGGGTAGGGGTTTATTATCGTGCAG
GGCGTAAACCTAGATGGCAAAATGCAGTTTATTGTCTTCAGAATCAGAGTCGGTACATGATTCATCTTGCTGGTTTAACC
AGTTTGGCTCAGCAGGGCAAATCGCATTACCTACAGTTTGCACCAGACTCTATTTGGATAAACGTTCAAGCAAAAACGAT
TTTACCAAAGTGGTTTAAAAATTTTCCAGAATTAGTTATTTCTGATGAACTAATACCAAATTGGGAAATCATTAAAACAT
CCAAGTTAAATGCTCAGGATGAGAATGACTTAACTACTATAGAAGTAGATGGGATTAAGTTAAAAGCAAGTAATCAAGAG
TTAGCCGCATTCGAGGTGCTGGAGGCTGTACCTTCCACTTTGTCATTTGAGCATGCAGCAGAGCTTTTTCAAGGATTGGT
AAACTTAAGTCCACGAAAAGTAGAACATATTTTAAAAAGAAGCGGATCTATTCAAACGAACCGACTTTTTTTATTTTTGG
GTCATTTCTATAAACATCCATGGGCTGAACGGGTGAATGAGTCCTTAATTAATCTTGGTGCAGGTAAGCGTCAAATAGTC
CCTGATGGAAAATTAGAAAAACAATATCAAATCACCGTTCCAGAACAATTTATAAGTAGCGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A128I8L5