Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 77247..78050 Replicon plasmid pSLy4
Accession NZ_CP015916
Organism Escherichia coli strain 210205630 isolate 210205630

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag A9C00_RS26970 Protein ID WP_048213819.1
Coordinates 77520..78050 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag A9C00_RS26965 Protein ID WP_048213818.1
Coordinates 77247..77516 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C00_RS30020 73588..74273 + 686 Protein_76 IS3 family transposase -
A9C00_RS26955 74946..76454 + 1509 WP_065304502.1 group II intron reverse transcriptase/maturase -
A9C00_RS29195 76535..77032 + 498 Protein_78 IS3 family transposase -
A9C00_RS26965 77247..77516 + 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
A9C00_RS26970 77520..78050 + 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
A9C00_RS26975 78135..79139 - 1005 WP_048213820.1 hypothetical protein -
A9C00_RS26980 79144..79389 - 246 WP_048213821.1 hypothetical protein -
A9C00_RS26985 79959..80309 + 351 WP_000493379.1 hypothetical protein -
A9C00_RS26990 80353..81042 + 690 WP_065304509.1 hypothetical protein -
A9C00_RS26995 81039..81827 + 789 WP_000016498.1 site-specific integrase -
A9C00_RS27000 82007..82651 + 645 WP_001144036.1 ParA family protein -
A9C00_RS27005 82738..83046 + 309 WP_000048808.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..100096 100096


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T63936 WP_048213819.1 NZ_CP015916:77520-78050 [Escherichia coli]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T63936 NZ_CP015916:77520-78050 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGCCAGCACAACAGTAAGATTTTGCGTGGCTATCTGCTCGTGACGAGAGAGG
ATAAACCACGTGTTATGGGGTACTACACGCTGTCTGGCAGCTGCTTTGAGAAAACACTTCTACCCAGCAAAACGCAACAA
AAGCGCGTCCCCTACAAAAATGTGCCGAGCGTGACTTTAGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCTATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGCCTGGGTTTTATACAGTTAAAAGAAGAAAACAGTAATTCGCTC
TTCTACCCGACAAAATCTGTTGAAGAATTGTTCGAAGTAAAAGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT63936 WP_048213818.1 NZ_CP015916:77247-77516 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT63936 NZ_CP015916:77247-77516 [Escherichia coli]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCCTGGAACCTGGTTATGGATGCAATCACTAATCCGCCAGTACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References