Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 95750..96351 Replicon plasmid pSLy2
Accession NZ_CP015914
Organism Escherichia coli strain 210205630 isolate 210205630

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag A9C00_RS25775 Protein ID WP_001216034.1
Coordinates 95971..96351 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag A9C00_RS25770 Protein ID WP_001190712.1
Coordinates 95750..95971 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C00_RS25760 92743..94014 - 1272 WP_023142242.1 restriction endonuclease subunit S -
A9C00_RS25765 94011..95567 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
A9C00_RS25770 95750..95971 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A9C00_RS25775 95971..96351 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
A9C00_RS25780 96356..96535 + 180 WP_001513661.1 hypothetical protein -
A9C00_RS25785 96563..96922 + 360 WP_001513660.1 hypothetical protein -
A9C00_RS25790 96846..97259 + 414 Protein_105 DDE-type integrase/transposase/recombinase -
A9C00_RS25795 97209..97526 - 318 WP_001513659.1 hypothetical protein -
A9C00_RS25800 97754..98734 - 981 WP_001567628.1 IS5-like element IS5 family transposase -
A9C00_RS25805 98978..100381 + 1404 WP_065304468.1 S-methylmethionine permease -
A9C00_RS25810 100368..101300 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid oqxB / oqxA / blaTEM-1B - 1..129035 129035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T63931 WP_001216034.1 NZ_CP015914:95971-96351 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T63931 NZ_CP015914:95971-96351 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT63931 WP_001190712.1 NZ_CP015914:95750-95971 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT63931 NZ_CP015914:95750-95971 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References