Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 7352..7887 Replicon plasmid pSLy2
Accession NZ_CP015914
Organism Escherichia coli strain 210205630 isolate 210205630

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag A9C00_RS25280 Protein ID WP_000222766.1
Coordinates 7352..7639 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag A9C00_RS25285 Protein ID WP_001132900.1
Coordinates 7636..7887 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C00_RS25255 4935..5435 + 501 Protein_4 Tn3 family transposase -
A9C00_RS25260 5469..5747 + 279 Protein_5 integrase core domain-containing protein -
A9C00_RS25275 6049..7277 + 1229 WP_113706755.1 IS3 family transposase -
A9C00_RS25280 7352..7639 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A9C00_RS25285 7636..7887 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A9C00_RS25290 8853..9710 - 858 WP_039002220.1 incFII family plasmid replication initiator RepA -
A9C00_RS25295 9703..9777 - 75 WP_061357229.1 RepA leader peptide Tap -
A9C00_RS25300 10009..10266 - 258 WP_001541895.1 replication regulatory protein RepA -
A9C00_RS25305 10534..10740 - 207 WP_001351576.1 type I toxin-antitoxin system Hok family toxin -
- 10727..10788 + 62 NuclAT_1 - -
- 10727..10788 + 62 NuclAT_1 - -
- 10727..10788 + 62 NuclAT_1 - -
- 10727..10788 + 62 NuclAT_1 - -
A9C00_RS25310 11130..11342 - 213 WP_001541896.1 ANR family transcriptional regulator -
A9C00_RS25315 11477..12037 - 561 WP_000139310.1 fertility inhibition protein FinO -
A9C00_RS25320 12092..12835 - 744 WP_065304457.1 conjugal transfer pilus acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid oqxB / oqxA / blaTEM-1B - 1..129035 129035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T63921 WP_000222766.1 NZ_CP015914:c7639-7352 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T63921 NZ_CP015914:c7639-7352 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT63921 WP_001132900.1 NZ_CP015914:c7887-7636 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT63921 NZ_CP015914:c7887-7636 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References