63920

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 15380..15873 Replicon plasmid pSLy1
Accession NZ_CP015913
Organism Escherichia coli strain 210205630 isolate 210205630

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag A9C00_RS24945 Protein ID WP_001230709.1
Coordinates 15380..15634 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag A9C00_RS24950 Protein ID WP_000151456.1
Coordinates 15631..15873 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C00_RS24910 10954..11115 + 162 WP_001366886.1 type I toxin-antitoxin system Hok family toxin -
A9C00_RS24915 11265..11861 + 597 WP_000125165.1 hypothetical protein -
A9C00_RS31010 11878..12162 - 285 WP_001162371.1 hypothetical protein -
A9C00_RS24925 12236..12901 - 666 WP_000532149.1 P-loop NTPase -
A9C00_RS24930 13489..13818 + 330 WP_000855119.1 hypothetical protein -
A9C00_RS24935 14222..14737 + 516 WP_001025393.1 J domain-containing protein -
A9C00_RS24940 14841..15383 + 543 WP_001083904.1 hypothetical protein -
A9C00_RS24945 15380..15634 + 255 WP_001230709.1 hypothetical protein Toxin
A9C00_RS24950 15631..15873 + 243 WP_000151456.1 hypothetical protein Antitoxin
A9C00_RS24960 16444..16737 + 294 WP_000673428.1 hypothetical protein -
A9C00_RS24965 17154..17336 - 183 WP_024135751.1 hypothetical protein -
A9C00_RS24970 17894..18076 + 183 WP_000833470.1 type II toxin-antitoxin system HicA family toxin -
A9C00_RS24975 18101..18538 + 438 WP_000466318.1 type II toxin-antitoxin system HicB family antitoxin -
A9C00_RS24980 18669..19109 - 441 WP_052993860.1 hypothetical protein -
A9C00_RS24985 19129..19728 - 600 WP_021500050.1 hypothetical protein -
A9C00_RS24990 19775..20476 - 702 WP_000280995.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..65888 65888
- flank IS/Tn - - 9287..10435 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T63920 WP_001230709.1 NZ_CP015913:15380-15634 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T63920 NZ_CP015913:15380-15634 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT63920 WP_000151456.1 NZ_CP015913:15631-15873 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT63920 NZ_CP015913:15631-15873 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References