Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4134861..4135479 Replicon chromosome
Accession NZ_CP015912
Organism Escherichia coli strain 210205630

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A9C00_RS20430 Protein ID WP_001291435.1
Coordinates 4134861..4135079 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A9C00_RS20435 Protein ID WP_000344800.1
Coordinates 4135105..4135479 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C00_RS20395 4130151..4130723 + 573 WP_000779826.1 lipoprotein -
A9C00_RS20400 4130754..4131065 - 312 WP_000409911.1 MGMT family protein -
A9C00_RS20410 4131444..4131797 + 354 WP_000878141.1 DUF1428 family protein -
A9C00_RS20415 4131839..4133389 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A9C00_RS20420 4133553..4134023 - 471 WP_000136192.1 YlaC family protein -
A9C00_RS20425 4134139..4134690 - 552 WP_000102564.1 maltose O-acetyltransferase -
A9C00_RS20430 4134861..4135079 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A9C00_RS20435 4135105..4135479 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A9C00_RS20440 4136025..4139174 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
A9C00_RS20445 4139197..4140390 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T63918 WP_001291435.1 NZ_CP015912:c4135079-4134861 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T63918 NZ_CP015912:c4135079-4134861 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT63918 WP_000344800.1 NZ_CP015912:c4135479-4135105 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT63918 NZ_CP015912:c4135479-4135105 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACAAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References