Detailed information of TA system TA063917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4100565..4101402
• Replicon: chromosome
• Accession: NZ_CP015912
• Organism: Escherichia coli strain 210205630

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: A9C00_RS20255
• Protein ID: WP_000227784.1
• Coordinates: 4100565..4101107 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: A9C00_RS20260
• Protein ID: WP_001297137.1
• Coordinates: 4101091..4101402 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9C00_RS20235	4096104..4097015	-	912	WP_000705865.1	2-dehydropantoate 2-reductase	-
A9C00_RS20240	4097183..4097674	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
A9C00_RS20245	4097802..4099166	-	1365	WP_001000960.1	MFS transporter	-
A9C00_RS20250	4099574..4100509	+	936	WP_065304348.1	sel1 repeat family protein	-
A9C00_RS20255	4100565..4101107	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
A9C00_RS20260	4101091..4101402	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
A9C00_RS20265	4101587..4102477	-	891	WP_000971336.1	heme o synthase	-
A9C00_RS20270	4102489..4102818	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
A9C00_RS20275	4102818..4103432	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
A9C00_RS20280	4103422..4105413	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
A9C00_RS20285	4105435..4106382	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T63917 WP_000227784.1 NZ_CP015912:c4101107-4100565 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T63917 NZ_CP015912:c4101107-4100565 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT63917 WP_001297137.1 NZ_CP015912:c4101402-4101091 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT63917 NZ_CP015912:c4101402-4101091 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3