Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1482832..1483457 Replicon chromosome
Accession NZ_CP015912
Organism Escherichia coli strain 210205630

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag A9C00_RS07455 Protein ID WP_000911329.1
Coordinates 1482832..1483230 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag A9C00_RS07460 Protein ID WP_000450524.1
Coordinates 1483230..1483457 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C00_RS07435 1478710..1478910 + 201 WP_000383836.1 YpfN family protein -
A9C00_RS07440 1479020..1479718 - 699 WP_000679812.1 esterase -
A9C00_RS07445 1479792..1481807 - 2016 WP_000829335.1 tRNA cytosine(34) acetyltransferase TmcA -
A9C00_RS07450 1481822..1482685 - 864 WP_001267498.1 neutral zinc metallopeptidase -
A9C00_RS07455 1482832..1483230 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
A9C00_RS07460 1483230..1483457 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
A9C00_RS07465 1483612..1484325 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
A9C00_RS07475 1484538..1485572 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
A9C00_RS07480 1485589..1486467 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
A9C00_RS07485 1486613..1487185 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
A9C00_RS07490 1487185..1487655 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) - 1420857..1554512 133655


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T63907 WP_000911329.1 NZ_CP015912:c1483230-1482832 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T63907 NZ_CP015912:c1483230-1482832 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT63907 WP_000450524.1 NZ_CP015912:c1483457-1483230 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT63907 NZ_CP015912:c1483457-1483230 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References