Detailed information of TA system TA063905

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 959776..960611
• Replicon: chromosome
• Accession: NZ_CP015912
• Organism: Escherichia coli strain 210205630

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: A9C00_RS04935
• Protein ID: WP_000854752.1
• Coordinates: 960234..960611 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: A9C00_RS04930
• Protein ID: WP_065304171.1
• Coordinates: 959776..960144 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9C00_RS04895	956612..957067	+	456	WP_000581504.1	hypothetical protein	-
A9C00_RS04900	957146..957379	+	234	WP_001119729.1	DUF905 family protein	-
A9C00_RS04905	957479..958297	+	819	WP_001234620.1	DUF945 domain-containing protein	-
A9C00_RS04910	958352..958837	+	486	WP_000849582.1	antirestriction protein	-
A9C00_RS04915	958853..959329	+	477	WP_001347688.1	RadC family protein	-
A9C00_RS04920	959392..959613	+	222	WP_000692329.1	DUF987 domain-containing protein	-
A9C00_RS04930	959776..960144	+	369	WP_065304171.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A9C00_RS04935	960234..960611	+	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
A9C00_RS04940	960608..960811	+	204	WP_000761705.1	hypothetical protein	-
A9C00_RS30140	960824..960937	+	114	WP_001161660.1	DUF957 domain-containing protein	-
A9C00_RS30925	961753..962124	+	372	WP_001295631.1	IS110 family transposase	-
A9C00_RS04950	962578..962760	+	183	WP_024167529.1	ethanolamine utilization protein	-
A9C00_RS04955	962861..963190	-	330	WP_000450409.1	DUF496 family protein	-
A9C00_RS04960	963362..964420	-	1059	WP_001200892.1	FUSC family protein	-
A9C00_RS04965	964618..965091	-	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T63905 WP_000854752.1 NZ_CP015912:960234-960611 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T63905 NZ_CP015912:960234-960611 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13438.33 Da
• Isoelectric Point: 6.3139

>AT63905 WP_065304171.1 NZ_CP015912:959776-960144 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT63905 NZ_CP015912:959776-960144 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCATGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4