Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 325320..325958 Replicon chromosome
Accession NZ_CP015912
Organism Escherichia coli strain 210205630

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A9C00_RS01690 Protein ID WP_000813794.1
Coordinates 325320..325496 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A9C00_RS01695 Protein ID WP_001270286.1
Coordinates 325542..325958 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C00_RS01670 320939..322153 - 1215 WP_071961377.1 BenE family transporter YdcO -
A9C00_RS01675 322206..322742 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
A9C00_RS01680 322815..324776 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A9C00_RS01685 324868..325098 - 231 WP_000494244.1 YncJ family protein -
A9C00_RS01690 325320..325496 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A9C00_RS01695 325542..325958 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A9C00_RS01700 326037..327443 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
A9C00_RS01705 327688..328833 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
A9C00_RS01710 328851..329864 + 1014 WP_065304068.1 ABC transporter ATP-binding protein -
A9C00_RS01715 329865..330806 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 319751..320899 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T63895 WP_000813794.1 NZ_CP015912:325320-325496 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T63895 NZ_CP015912:325320-325496 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT63895 WP_001270286.1 NZ_CP015912:325542-325958 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT63895 NZ_CP015912:325542-325958 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References